Product Category
DNA Damage Repair Enzymes
DNA Damage Repair Enzymes are necessary for the repair of errors introduced during DNA replication or recombination or as a result of treatment with exogenous genotoxic agents. These enzymes can be used in vitro to detect specific forms of DNA damage or for repair assays. The DNA damage repair substrates are oligonucleotides that contain specific mutations which are recognized and cleaved by some of the following DNA repair enzymes. These substrates can serve as positive controls for DNA repair assays.
DNA Damage Repair Enzymes
- Mouse Aag (Catalog # 4090-100-EB)
Specificity: Catalyzes the excision of damaged DNA bases, including 3-methyladenine, 3-methylguanine, 7-methylguanine, hypoxanthine, 1,N6-ethenoadenine, and a number of other substrates (see product insert).
Research Application: DNA methylation and oxidative damage
- Human DNA Polymerase beta (Catalog # 4020-100-EB; 4020-500-EB)
Specificity: Fills small gaps and nicks in the DNA, catalyzes DNA synthesis after nucleotide excision repair, and releases 5' terminal deoxyribose phosphate residues from incised AP sites.
Research Application: DNA gap-filling
- E. coli Endonuclease III (Catalog # 4045-01K-EB; 4045-05K-EB)
Specificity: Releases bases damaged by UV light, ionizing radiation, osmium tetroxide, or acid. Catalyzes the excision of cis- and trans- thymine glycol, 5,6-dihydrothymine, 5,6-dihydroxydihydrothymine, 5-hydroxy-5-methylhydantoin, 6-hydroxy-5,6-dihydropyrimidines, 5-hydroxycytosine and 5-hydroxyuracil, 5-hydroxy-6-hydrothymine, 5,6-dihydrouracil, 5-hydroxy-6-hydrouracil, AP sites, uracil glycol, methyltartronylurea, alloxan, and urea.
Research Application: Radiation, oxidation, and UV damage
- E. coli Endonuclease IV (Catalog # 4050-100-EB; 4050-500-EB)
Specificity: Catalyzes the removal of phosphoglycoaldehyde, deoxyribose-5-phosphate, 4-hydroxy-2-pentanal, and phosphate groups from the 3' ends of DNA. The preparation is free of exonuclease activity.
Research Application: AP sites, oxidizing agents (bleomycin, tert-butyl hydroperoxide), alkylating agents (MMS, mitomycin)
- Chorella virus Endonuclease V (Catalog # 4065-100-EB)
Specificity: Recognizes cis-syn and trans-syn cyclobutane pyrimidine dimers and AP sites with equal efficiency. The enzyme cleaves AP sites on both double-, and single-stranded DNA.
Research Application: UV damage
- T4 Endonuclease V (Catalog # 4055-100-EB)
Specificity: Recognizes cis-syn cyclobutane pyrimidine dimers and AP sites.
Research Application: UV damage
- Human FEN-1 (Catalog # 4120-100-EB)
Specificity: Catalyzes the excision of DNA branch structures formed during replication of lagging strand DNA, and endonucleolytic cleavage of branch structures formed during DNA double strand break end-joining. Cleaves DNA flap structures and has a 5'-3' exonuclease activity specific for recessed 5' ends.
Research Application: Double strand break repair, base excision repair
- E. coli Fpg (Catalog # 4040-100-EB)
Specificity: Catalyzes the excision of multiple forms of damaged bases, including 8-oxoguanine, 5-hydroxycytosine, 5-hydroxyuracil, aflatoxin-bound imidazole ring-opened guanine, imidazole ring-opened N-2-aminofluorene-C8-guanine, and open ring forms of 7-methylguanine.
Research Application: Radiation and oxidative damage
- E. coli Mug (Catalog # 4125-100-EB)
Specificity: Catalyzes the removal of uracil in U:G mismatches in double-stranded oligonucleotides, and the excision of 3, N4-ethenocytosine (eC) in eC:G mismatches in double-, or single-stranded oligonucleotides.
Research Application: Deamination of cytosine
- E. coli MutY DNA Glycosylase (Catalog # 4000-500-EB)
Specificity: Acts with Fpg to prevent the potentially mutagenic consequences of 8-oxo-dG lesions. Catalyzes the removal of adenine from 8-oxo-dG:A mispairs to initiate base excision repair.
Research Application: 8-oxo-dG:A mismatches and oxidative damage
- Human OGG1 (Catalog # 4130-100-EB)
Specificity: Catalyzes the removal of 8-oxoguanine and DNA-containing formamidopyrimidine moieties. 8-oxoguanine mispairs with adenine during replication, and gives rise to G:C to T:A transversions.
Research Application: Radiation and oxidative damage
- PARP High Specific Activity(Catalog # 4668-100-01; 4668-500-01)
Specificity: Modifies proteins post-translationally by poly ADP ribosylation using nicotinamide adenine dinucleotide (NAD) as a substrate. Poly ADP ribosylation of proteins occurs by the addition of an ADP-ribose to glutamic acid residues.
Research Application: Assessment of damaged DNA, measurement of PARP inhibition, identification of PARP inhibitors or activators
- M. thermoautotrophicum TDG (Catalog # 4070-500-EB)
Specificity: Recognizes T:G mismatches in duplex DNA and cleaves the strand with the T, while the opposite strand is not cleaved. It also recognizes G:G mismatches if at least one of the neighboring bases is an adenine or a thymine and nicks one strand or the other.
Research Application: T:G, G:G mismatches and deamination of 5-methyl-cytosine
- E. coli Uracil DNA N-Glycosylase (Catalog # 4025-100-EB)
Specificity: Catalyzes the removal of uracil from double-, or single-stranded DNA to generate an AP site. Also recognizes 5-fluorouracil, 5-hydroxy-uracil, and isodialuric acid.
Research Application: Removes uracil from DNA