R&D Systems Inc.
Tocris Bioscience
Boston Biochem
 

Product Category

Feeder Cells for Embryonic & Induced Pluripotent Stem Cells

Irradiated Mouse Embryonic Fibroblasts (iMEF; Catalog # PSC001)

Mouse embryonic fibroblasts are used to maintain and expand pluripotent human embryonic stem cells (hES) in an undifferentiated state or to induce differentiation along somatic or germ cell lineages. R&D Systems iMEFs (Catalog # PSC001) are mitotically inactivated by irradiation rather than by use of pharmacological inhibitors, thus eliminating possible exposure of your ES cells to toxic agents.

Each iMEF lot is confirmed negative for mycoplasma contamination using the MycoProbe® mycoplasma detection kit (Catalog # CUL001B) and negative for microbial contamination. Furthermore, each lot is tested for its ability to support undifferentiated growth of the BG01V human embryonic stem cells as assessed by Oct-3/4 and SSEA-4 expression.

Features

  • Consistent
  • Ready-to-use
  • Mitotically inactivated by irradiation
  • Maintains pluripotency of human ES cells
  • Negative for mycoplasma and microbial contamination

A
 B
C
 D
Human Embryonic Stem Cells (BG01V Human Embryonic Stem Cells) Cultured on iMEFs were Assessed Using Markers of Pluripotency. BG01V colonies (highlighted by arrows) growing in culture on iMEFs (A) were analyzed for markers of pluripotency after 3 passages. Cells were evaluated for SSEA-4 expression by flow cytometry (B) using PE-conjugated anti-human SSEA-4 (Catalog # FAB1435P; filled histogram) or isotype control antibody (open histogram). BG01V colonies were incubated with anti-human Oct-3/4 (C, Catalog # AF1759) or anti-human Nanog (D), Catalog # AF1997) followed by staining with NorthernLights™ 557-conjugated anti-goat secondary antibody (Catalog # NL001).

BG01V Cells are licensed from BresaGen, Inc.
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