| Black microplates are pre-coated with protease-specific antibody. The sample is added allowing both pro- and active forms of the enzyme to bind the immobilized antibody. Unbound proteases are removed following wash steps. |
A protease-specific peptide substrate (green & purple) is added. The substrate features a fluorophore (green) and quencher molecule (purple) on opposite sides of the prospective cleavage site. |