Product Category
Fluorokine MAP Human Cardiac Panel B
Base Kit: LUCB000 Human Fluorokine MAP Base Kit, Cardiac Panel B
This Base Kit is compatible with the following analyte-specific bead sets:
| Analyte |
Bead Set Catalog Number |
Serum |
EDTA Plasma |
Heparin Plasma |
Sensitivity
(pg/mL) |
High Standard Value* (pg/mL) |
Microparticle Region |
| C-Reactive Protein |
LUCB1707 |
X |
X |
X |
39.0 |
105,000 |
4 |
| Cystatin C |
LUCB1196 |
X |
X |
X |
70.4 |
308,000 |
8 |
| Myeloperoxidase** |
LUCB3174 |
X |
X |
X |
38.8 |
92,000 |
12 |
| P-Selectin |
LUCB137 |
X |
X |
X |
16.5 |
41,300 |
21 |
| Serpin E1/PAI-1 |
LUCB1786 |
X |
X |
X |
0.84 |
6,300 |
26 |
| TIMP-1 |
LUCB970 |
X |
X |
X |
5.05 |
11,300 |
37 |
*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.
**Myeloperoxidase is present in neutrophil granules and is released upon neutrophil exposure to activated platelets. Therefore, to measure circulating levels of myeloperoxidase, platelet-free plasma should be collected for measurement. It should be noted that many protocols for plasma preparation, including procedures recommended by the Clinical and Laboratory Standards Institute(CLSI), result in incomplete removal of platelets from blood. This will cause variable and irreproducible results for assays of factors released by platelet activation.
|
Sample Preparation
Total prepared sample volume needed per well is 50 microliters.
Serum and plasma samples require a 100-fold dilution. For testing samples in duplicate, a suggested 100-fold dilution is 10 microliters of sample + 990 microliters of Calibrator Diluent RD6-56.