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• Product Insert PDF
• Assays for analytes represented in the Human Angiogenesis Panel A

Base Kit: LAN000 Human Fluorokine MAP Base Kit, Panel A
This Base kit is compatible with the following analyte-specific bead sets:

Analyte	Catalog Number	Cell Culture	Serum	EDTA Plasma	Heparin Plasma	Urine	Human Milk	High Standard Value* (pg/mL)	Microparticle Region
Angiogenin	LAN265	X	X	X	X	X	X	10,400	05
Angiopoietin-1	LAN923	X	X	X	X	X	X	149,000	22
Endostatin	LAN1098	X	X	X	X	X	X	90,500	18
FGF acidic	LAN232	X	X	X	X	X	X	15,800	33
FGF basic	LUH233	X	X	X		X	X	2200	54
PDGF-AA	LAN221	X	X	X	X	X	X	7350	51
PDGF-BB	LAN220	X	X	X	X	X	X	21,800	37
PlGF	LAN264	X	X	X	X	X	X	11,300	34
Thrombospondin-2	LAN1635	X	X	X	X	X	X	230,000	59
VEGF	LUH293	X	X	X	X	X	X	2400	52
VEGF-D	LAN622	X	X	X	X	X	X	87,300	66
*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

Sample Preparation

Total prepared sample volume needed per well is 100 microliters.

Serum, plasma*, and milk samples require a 5-fold dilution. A suggested 5-fold dilution is 50 microliters of sample + 200 microliters of Calibrator Diluent RD6-49. When assaying Angiogenin, serum and plasma samples must be further diluted 10-fold to a final 50-fold dilution and milk samples an additional 4-fold dilution to a final 20-fold dilution. The minimum starting volume recommended for sample dilution is 10 microliters. While 100 microliters of prepared (diluted) sample is added to each well, the actual serum/plasma volume in each well will be less than 10 microliters. Pipetting less than 10 microliters of sample is not recommended due to potential pipetting inaccuracy. Samples may be further diluted if necessary to bring sample data within the standard curve range.

Cell culture supernates and urine samples require a 5-fold dilution. A suggested 5-fold dilution is 50 microliters of sample + 200 microliters of Calibrator Diluent RD6-49 (1.5X). Samples may be further diluted if necessary to bring sample data within the standard curve range.

*Angiopoietin-1, PDGF-AA, and PDGF-BB are present in platelet granules and are released upon platelet activation. Therefore, to measure circulating levels of these factors, platelet-free plasma should be collected for measurement.