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• Product Insert PDF
• Assays for analytes represented in the Human Cytokine Panel A

Base Kit: LUH000 Human Fluorokine MAP Base Kit, Panel A
This Base kit is compatible with the following analyte-specific bead sets:

Analyte	Catalog Number	Cell Culture	Serum	EDTA Plasma	Heparin Plasma	Sensitivity (pg/mL)	High Standard Value* (pg/mL)	Microparticle Region
CCL2/MCP-1	LUH279	X	X	X	X	0.47	2400	78
CCL3/MIP-1 alpha	LUH270	X	X	X	X	1.45	12,000	59
CCL4/MIP-1 beta	LUH271	X	X	X	X	0.72	4200	74
CCL5/RANTES	LUH278	X	X	X	X	1.91	1700	80
CXCL8/IL-8	LUH208	X	X	X	X	1.97	2500	36
CXCL5/ENA-78	LUH254	X	X	X	X	4.14	4000	37
FGF basic	LUH233	X	X	X		4.91	2200	54
G-CSF	LUH214	X	X	X	X	1.48	2400	58
GM-CSF	LUH215C	X	X	X	X	1.98	1800	11
IFN-gamma	LUH285	X	X	X	X	1.27	1900	75
IL-1 alpha/IL-1F1	LUH200B	X	X	X	X	0.36	2600	05
IL-1ra/IL-1F3	LUH280	X	X	X	X	10.91	4100	16
IL-1 beta/IL-1F2	LUH201	X	X	X	X	0.57	1920	06
IL-2	LUH202	X	X	X	X	2.23	2320	17
IL-4	LUH204	X	X	X	X	4.46	2200	21
IL-5	LUH205	X	X	X	X	0.71	1300	09
IL-6	LUH206	X	X	X	X	1.11	2400	32
IL-10	LUH217	X	X	X	X	0.30	2320	50
IL-17	LUH317	X	X	X	X	1.10	2100	20
TNF-alpha	LUH210	X	X	X	X	1.50	3900	77
Tpo	LUH288	X	X	X	X	9.94	10,000	34
VEGF	LUH293	X	X	X	X	1.84	2400	52
*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

Sample Preparation

Total prepared sample volume needed per well is 50 microliters.

Serum and plasma samples require a 4-fold dilution. For testing samples in duplicate, a suggested 4-fold dilution is 30 microliters of sample + 90 microliters of Calibrator Diluent RD6-40. When assaying RANTES, serum/plasma samples must be further diluted 25-fold to a final 100-fold dilution. A suggested 100-fold dilution is 10 microliters of the 4-fold diluted sample + 240 microliters of Calibrator Diluent RD6-40. The minimum starting serum/plasma volume recommended for sample dilution is 10 microliters. While 50 microliters of prepared (diluted) sample is added to each well, the actual serum/plasma volume in each well will be less than 10 microliters. Pipetting less than 10 microliters of serum/plasma is not recommended due to potential pipetting inaccuracy. Samples may be further diluted if necessary to bring sample data within the standard curve range.

Cell culture supernates can be run undiluted with no matrix effects or interference. Maximum sample requirement per well is 50 microliters. Samples may be further diluted if necessary to bring sample data within the standard curve range.