R&D Systems Inc.
Tocris Bioscience
Boston Biochem
 

Product Category

Fluorokine MAP Mulitplex Human TIMP 4-plex Kit

Cell Culture Serum EDTA or
Heparin Plasma		 Urine Saliva Human Milk Sensitivity
(pg/mL)		 High Standard
Value* (pg/mL)		 Microparticle Region
TIMP-1 X X X X X X 3.43 10,000 9
TIMP-2 X X X X X X 40.1 39,000 33
TIMP-3 X X X X X X 253 100,000 34
TIMP-4 X X X X X X 3.8 4000 74
*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the kit.

Sample Preparation

  • Total prepared sample volume needed per well is 50 microliters.
  • Serum and plasma samples require a 50-fold dilution. For testing samples in duplicate, a suggested 50-fold dilution is 10 microliters of sample + 490 microliters of Calibrator Diluent RD6-48.
  • Cell culture supernate samples require a 10-fold dilution. For testing samples in duplicate, a suggested 10-fold dilution is 25 microliters of sample + 225 microliters of Calibrator Diluent RD6-48.
  • Urine samples require a 5-fold dilution. For testing samples in duplicate, a suggested 5-fold dilution is 50 microliters of sample + 200 microliters of Calibrator Diluent RD6-48.
  • Saliva samples require a 100-fold dilution. For testing samples in duplicate, a suggested 100-fold dilution is 10 microliters of sample + 990 microliters of Calibrator Diluent RD6-48.
  • Human milk samples require a 200-fold dilution. For testing samples in duplicate, a suggested 200-fold dilution is 10 microliters of sample + 190 microliters of Calibrator Diluent RD6-48. After this initial 20-fold dilution, combine 20 microliters of the diluted sample + 180 microliters of Calibrator Diluent RD6-48 for a final 200-fold dilution.

Assays for analytes represented in the Human TIMP 4-plex

Quantikine ELISA Kits DuoSet ELISA Development Systems
TIMP-1 DTM100 DY970
TIMP-2 DTM200 DY971
TIMP-3 DY973
TIMP-4 DTM400 DY974
Search Technical Information:
Search