Base Kit: LOB000 Human Fluorokine MAP Base Kit, Obesity
| Analyte |
Catalog Number |
Cell Culture |
Serum |
EDTA Plasma |
Heparin Plasma |
Sensitivity (pg/mL) |
High Standard Value* (pg/mL) |
Microparticle Region |
Adiponectin/
Acrp30 |
LOB1065 |
x |
x |
x |
x |
19.8 |
250,000 |
2 |
| C-Reactive Protein |
LOB1707 |
x |
x |
x |
x |
1.9 |
23,500 |
8 |
| CCL2/MCP-1 |
LUH279 |
x |
x |
x |
x |
2.54 |
2000 |
78 |
Complement Factor D/
Adipsin |
LOB1824 |
x |
x |
x |
x |
9.5 |
86,000 |
11 |
| IL-6 |
LUH206 |
x |
x |
x |
x |
1.11 |
2400 |
32 |
| IL-10 |
LUH217 |
x |
x |
x |
x |
0.30 |
2320 |
50 |
| Leptin (OB) |
LUB398 |
x |
x |
x |
x |
20.2 |
59,000 |
31 |
| Resistin |
LOB1359 |
x |
x |
x |
x |
7.3 |
32,700 |
12 |
| Serpin E1/ PAI-1 |
LOB1786 |
x |
x |
x |
x |
2.9 |
10,100 |
10 |
| TNF-alpha |
LUH210 |
x |
x |
x |
x |
0.92 |
2140 |
77 |
| *A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit. |
Sample Preparation
Total prepared sample volume needed per well is 50 microliters.
Serum and plasma samples require a 4-fold dilution. For testing samples in duplicate, a suggested 4-fold dilution is 30 microliters of sample + 90 microliters of Calibrator Diluent RD6-46. For Adiponectin, CRP, Factor D, and Serpin E1, serum and plasma samples may require up to a 100-fold dilution. A suggested 100-fold dilution is 10 microliters of the 4-fold diluted sample + 240 microliters of Calibrator Diluent RD6-46. If expected sample values are unknown, it is recommended to evaluate both a 4-fold and a 100-fold sample dilution. The minimum starting serum/plasma volume recommended for sample dilution is 10 microliters. While 50 microliters of prepared (diluted) sample is added to each well, the actual serum/plasma volume in each well will be less than 10 microliters. Pipetting less than 10 microliters of serum/plasma is not recommended due to potential pipetting inaccuracy. Samples may be further diluted if necessary to bring sample data within the standard curve range.
Cell culture supernates can be run undiluted with no matrix effects or interference. Maximum sample requirement per well is 50 microliters. Samples may be further diluted if necessary to bring sample data within the standard curve range.