Product Category
Human Kidney Biomarker Array
Introduction
R&D Systems Proteome Profiler™ Human Kidney Biomarker Array Kit (Catalog # ARY019) is a rapid, sensitive, and economical tool to simultaneously detect the relative changes of 38 proteins in a single sample. No specialized equipment is necessary.
| Simultaneously detect the levels of these cell stress-related proteins in a single sample. |
| Adiponectin |
Fetuin A |
Renin |
| Aminopeptidase N |
GRO-alpha/CXCL1 |
Resistin |
| Angiotensinogen/Serpin A8 |
IL-1ra |
SCF |
| Annexin V |
IL-6 |
Serpin A3 |
| beta2-Microglobulin |
IL-10 |
TNF-alpha |
| Clusterin |
TIM-1/KIM-1/HAVCR |
TNF RI |
| CXCL16 |
Lipocalin-2/NGAL |
Trefoil Factor 3 |
| Cyr61/CCN |
MCP-1/CCL2 |
Thrombospondin-1 |
| Cystatin C |
MMP-9 |
TWEAK |
| DPPIV/CD26 |
Neprilysin |
uPA |
| EGF |
PSA/KLK-3 |
VCAM-1 |
| EGF R/ErbB1 |
RAGE |
VEGF-A |
| FABP1/L-FABP |
RBP-4 |
|
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Urine, cell culture supernatant, and cell or tissue lysate samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any biomarker/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of kidney biomarker bound. Chemiluminescence is detected in the same manner as a Western blot.
Kit Contents
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.