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• Human sReceptor Array Kit Non-Hematopoietic Panel Ordering Information

Introduction

R&D Systems Human Soluble Receptor Array Kit Non-Hematopoietic Panel (Catalog # ARY012) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 119 different soluble receptors expressed and released by non-hematopoietic cells in a single sample. No specialized equipment is necessary.

• Product Insert
• Troubleshooting Guide
• Common assays for analytes represented in the Human Common Analyte Array
• Assays for analytes represented in the Human sReceptor Array Kit Non-Hematopoietic Panel

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, serum, or cell lysate samples are diluted and incubated with each array part of the Non-Hematopoietic Panel. This panel is comprised of the Non-Hematopoietic Array and the Common Analytes Array, which is also used in the Hematopoietic Array Panel (R&D Systems, Catalog # ARY011). The membranes are washed to remove unbound material followed by incubation with their specific cocktail of biotinylated detection antibodies. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents* are added and a signal is produced in proportion to the amount of cytokine bound.

Kit Contents

• 2 Non-Hematopoeitic Array Membranes
• 2 Common Analyte Array Membranes
• 4-Well Multi-dish
• Array Buffers
• Wash Buffer
• Antibody Detection Cocktail
• Streptavidin-HRP
• Transparency Overlay Template
• Detailed Protocol

*Chemiluminescence detection substrate not included. For a complete list of the kit contents and necessary materials, please see the Materials Provided and Other Materials Required sections of the product insert.