Product Category
Human Phospho-Immunoreceptor Array Kit
Introduction
R&D Systems Human Phospho-Immunoreceptor Array (Catalog # ARY004) is a rapid, sensitive, and economical tool used to simultaneously detect the relative phosphorylation levels of 59 ITAM/ITIM-associated immunoreceptors, avoiding numerous immunoprecipitations and/or Western blots.
| Simultaneously detect the relative phosphorylation levels of these immunoreceptors in a single sample. |
| 2B4/SLAMF4 |
FcRH1/IRTA5 |
NKp46/NCR1 |
| BLAME/SLAMF8 |
FcRH2/IRTA4 |
NKp80/KLRF1 |
| BTLA |
FcRH4/IRTA1 |
NTB-A/SLAMF6 |
| CD3 epsilon |
FcRH5/IRTA2 |
PD-1 |
| CD5 |
ILT2/CD85j |
PECAM/CD31 |
| CD6 |
ILT3/CD85k |
SHIP-1 |
| CD28 |
ILT4/CD85d |
SHP-1 |
| CD84/SLAMF5 |
ILT5/CD85a |
SHP-2 |
| CD229/SLAMF3 |
ILT6/CD85e |
Siglec-2/CD22 |
| CEACAM-1 |
Integrin beta 3/CD61 |
Siglec-3/CD33 |
| CLEC-1 |
KIR2DL4 |
Siglec-5 |
| CLEC-2 |
LAIR-1 |
Siglec-7 |
| CRACC/SLAMF7 |
LAIR-2 |
Siglec-9 |
| CTLA-4/CD152 |
LMIR1/CD300A |
Siglec-10 |
| DCIR/CLEC4A |
LMIR2/CD300C |
SIRP-beta 1 |
| Dectin-1/CLEC7A |
LMIR3/CD300LF |
SLAM/CD150 |
| DNAM-1 |
LMIR6/CD300LE |
TREM-1 |
| Fc epsilon RII/CD23 |
MDL-1/CLEC5A |
TREM-2 |
| Fc gamma RIIA |
NKp30/NCR3 |
TREML1/TLT-1 |
| Fc gamma RIIIA/B |
NKp44/NCR2 |
|
General Assay Principle
Capture and control antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysates are incubated with the array membrane, which binds both phosphorylated and unphosphorylated immunoreceptors. A pan anti-phospho-tyrosine antibody conjugated to HRP is then used, followed by chemiluminescence detection.* See the product insert link below for details.
Alternative Total Immunoreceptor Profile Assay Principle
This kit can also be used to obtain a total immunoreceptor profile by using lysates prepared from cell-surface biotinylated cells and streptavidin-HRP ( Catalog # DY998) for detection. A biotinylated positive control for streptavidin-HRP detection is spotted on three corners of the array next to the phospho-tyrosine positive control. Details for this protocol can be found by clicking the link below.
Kit Contents
- 4 single-use nitrocellulose membranes spotted in duplicate with capture and control antibodies
- Buffers for sample lysis, blocking, antibody dilution, and washing
- Anti-phospho-tyrosine-HRP antibody
- Transparency overlay template
- Detailed insert with protocols
*Chemiluminescence detection substrate not included. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product insert ( PDF 168 KB).