Human Phospho-Immunoreceptor Array Kit
Human Phospho-Immunoreceptor Array Kit
Simultaneously detect the relative phosphorylation levels of these immunoreceptors in a single sample.
2B4/SLAMF4 FcRH1/IRTA5 NKp46/NCR1
BLAME/SLAMF8 FcRH2/IRTA4 NKp80/KLRF1
BTLA FcRH4/IRTA1 NTB-A/SLAMF6
CD3 epsilon FcRH5/IRTA2 PD-1
CD5 ILT2/CD85j PECAM/CD31
CD6 ILT3/CD85k SHIP-1
CD28 ILT4/CD85d SHP-1
CD84/SLAMF5 ILT5/CD85a SHP-2
CD229/SLAMF3 ILT6/CD85e Siglec-2/CD22
CEACAM-1 Integrin beta 3/CD61 Siglec-3/CD33
CLEC-1 KIR2DL4 Siglec-5
CLEC-2 LAIR-1 Siglec-7
CRACC/SLAMF7 LAIR-2 Siglec-9
CTLA-4/CD152 LMIR1/CD300A Siglec-10
DCIR/CLEC4A LMIR2/CD300C SIRP-beta 1
Dectin-1/CLEC7A LMIR3/CD300LF SLAM/CD150
DNAM-1 LMIR6/CD300LE TREM-1
Fc epsilon RII/CD23 MDL-1/CLEC5A TREM-2
Fc gamma RIIA NKp30/NCR3 TREML1/TLT-1
Fc gamma RIIIA/B NKp44/NCR2

Introduction

R&D Systems Human Phospho-Immunoreceptor Array (Catalog # ARY004) is a rapid, sensitive, and economical tool used to simultaneously detect the relative phosphorylation levels of 59 ITAM/ITIM-associated immunoreceptors, avoiding numerous immunoprecipitations and/or Western blots.

General Assay Principle

Capture and control antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysates are incubated with the array membrane, which binds both phosphorylated and unphosphorylated immunoreceptors. A pan anti-phospho-tyrosine antibody conjugated to HRP is then used, followed by chemiluminescence detection.* See the product insert link below for details.

Alternative Total Immunoreceptor Profile Assay Principle

This kit can also be used to obtain a total immunoreceptor profile by using lysates prepared from cell-surface biotinylated cells and streptavidin-HRP ( Catalog # DY998) for detection. A biotinylated positive control for streptavidin-HRP detection is spotted on three corners of the array next to the phospho-tyrosine positive control. Details for this protocol can be found by clicking the link below.

Kit Contents

  • 4 single-use nitrocellulose membranes spotted in duplicate with capture and control antibodies
  • Buffers for sample lysis, blocking, antibody dilution, and washing
  • Anti-phospho-tyrosine-HRP antibody
  • Transparency overlay template
  • Detailed insert with protocols

*Chemiluminescence detection substrate not included. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product insert ( PDF 168 KB).





RELATED INFORMATION
  • ITIM/ITAM Immunoreceptors & Related Molecules
  • Proteome Profiler Antibody Arrays