• Ordering Information & Pricing

Introduction

R&D Systems Human Phospho-Immunoreceptor Array (Catalog # ARY004) is a rapid, sensitive, and economical tool used to simultaneously detect the relative phosphorylation levels of 59 ITAM/ITIM-associated immunoreceptors, avoiding numerous immunoprecipitations and/or Western blots.

• Sample Data
• Troubleshooting Guide

General Assay Principle

Capture and control antibodies have been spotted in duplicate on nitrocellulose membranes. Cell lysates are incubated with the array membrane, which binds both phosphorylated and unphosphorylated immunoreceptors. A pan anti-phospho-tyrosine antibody conjugated to HRP is then used, followed by chemiluminescence detection.* See the product insert link below for details.

• Immunoreceptor array insert (168 KB PDF)

Alternative Total Immunoreceptor Profile Assay Principle

This kit can also be used to obtain a total immunoreceptor profile by using lysates prepared from cell-surface biotinylated cells and streptavidin-HRP ( Catalog # DY998) for detection. A biotinylated positive control for streptavidin-HRP detection is spotted on three corners of the array next to the phospho-tyrosine positive control. Details for this protocol can be found by clicking the link below.

• Cell-Surface Biotinylation Profile Protocol

Kit Contents

• 4 single-use nitrocellulose membranes spotted in duplicate with capture and control antibodies
• Buffers for sample lysis, blocking, antibody dilution, and washing
• Anti-phospho-tyrosine-HRP antibody
• Transparency overlay template
• Detailed insert with protocols

*Chemiluminescence detection substrate not included. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product insert ( PDF 168 KB).