Methylcellulose-based Reagents for Colony Forming Cell Assays
Methylcellulose-based Reagents for Colony Forming Cell Assays
CFU-E BFU-E
CFU-E BFU-E
CFU-G CFU-GM
CFU-G CFU-GM
CFU-GEMM CFU-M
CFU-GEMM CFU-M

Methylcellulose Stock Solution

(Catalog # HSC001)

Contains 2.8% methylcellulose in Iscove's MDM; can be used in both human and mouse hematopoietic stem cell research.

Human Methylcellulose Base Media

(Catalog # HSC002)

Contains all of the basic components with the exception of the cytokines required to perform CFC assays for hematopoietic stem cell research.

Human Methylcellulose Complete Media

(Catalog # HSC003)

A specially formulated media supplemented with recombinant human cytokines (GM-CSF, IL-3, SCF, and Epo) for routine assays of human clonogenic hematopoietic progenitors from human bone marrow, peripheral blood, cord blood, leukopheresis products, and purified CD34+ cells.

For the enumeration of:
  • CFU-E (colony-forming unit-erythroid)
  • BFU-E (burst-forming unit-erythroid)
  • CFU-G (colony-forming unit-granulocyte)
  • CFU-M (colony-forming unit- macrophage)
  • CFU-GM (colony-forming unit-granulocyte/macrophage)
  • CFU-GEMM (colony-forming unit-granulocyte/erythroid/macrophage/megakaryocyte)

Human Methylcellulose Complete Media without Epo

( Catalog # HSC004)

A specially formulated media supplemented with recombinant human cytokines (GM-CSF, IL-3, and SCF) for routine assays of human clonogenic hematopoietic progenitors from human bone marrow, peripheral blood, cord blood, leukopheresis products, and purified CD34+ cells.

For the enumeration of:
  • CFU-GM
  • CFU-G
  • CFU-M

Human Methylcellulose Enriched Media

( Catalog # HSC005)

A specially formulated media supplemented with recombinant human cytokines (GM-CSF, G-CSF, IL-3, IL-6, SCF, and Epo) optimized for use with purified CD34+ cells in the CFC assay at the end of the long-term culture-initiating cell (LTC-IC) assay.

For the enumeration of:
  • CFU-E
  • BFU-E
  • CFU-G
  • CFU-M
  • CFU-GM
  • CFU-GEMM




RELATED INFORMATION
  • Methylcellulose-based Reagents for Colony Forming Cell Assays