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Mosaic™ ELISA Human Inflammation Panel 1

The Mosaic™ ELISA Human Inflammation Panel 1 (Catalog # MEA004) is an excellent tool for the simultaneous quantitative determination of 8 different secreted mediators of inflammation in a single sample of cell culture supernate, serum, and plasma. Mosaic ELISA kits employ multiplex microarray technology to provide an accurate, efficient, and economical alternative to conducting multiple traditional ELISA experiments.

Antibody Well Map

A visualization of the spot layout per well in the Mosaic ELISA Human Inflammation Panel 1 (Catalog # MEA004). Each individual well is spotted with 8 different antibodies and a Reference Spot. Reference Spots provide a strong positive signal to allow easy visualization of well location for template alignment during data analysis.

Features

  • Detects 8 inflammation biomarkers in a single sample
  • An array in each well of a 96-well microplate
  • 320 data points per microplate (40 samples in duplicate plus 2 standard curves)
  • Utilizes small sample size (25-50 μL*)
  • Quantitative
  • Validated for cell culture supernates, serum, and plasma
  • 4.5 hour assay time
  • Amenable to automation

*Serum and plasma samples require a 2-fold dilution.

Kit Contents**

  • 96-well microplate with each well spotted with 8 capture antibodies & 1 reference spot
  • Cocktail of purified protein standards
  • Cocktail of biotinylated detection antibodies
  • Streptavidin-HRP
  • Diluent & Wash Buffers
  • Chemiluminescent Reagents**
  • Plate Covers


*Acquisition of data from Mosaic ELISAs requires a Digital Imaging System.

**Substrates 1 and 2 are comprised of TMA-6, a product of Lumigen, Inc., Southfield, Michigan, USA, and are covered by the following US Patent Numbers: 5,922,558 and 6,858,733 and International Patent Numbers: 733,086, 1,019,525, 2,300,071, 1,015,461, 2,002,352,881, ZL02805225.0, and 1,456,716