Product Category
Mosaic™ ELISA Human MMP Panel
The Mosaic ELISA Human MMP Panel (Catalog # MEA006) is an excellent tool for the simultaneous quantitative determination of 7 different matrix metalloproteinases (MMPs) in a single sample of cell culture supernate, serum, plasma, saliva, and urine. Mosaic ELISA kits employ multiplex microarray technology to provide an accurate, efficient, and economical alternative to conducting multiple traditional ELISA experiments.
| Antibody Well Map |
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A Visualization of the Spot Layout in Each Well of the Mosaic ELISA Human MMP Panel (Catalog # MEA006). Each individual well is spotted with 7 different capture antibodies and a Reference Spot. Reference Spots provide a strong positive signal to allow easy visualization of well location for template alignment during data analysis. |
Features
- Detects 7 MMPs in a single sample
- An array in each well of a 96-well microplate
- 287 data points per microplate (41 samples plus a standard curve in duplicate)
- Utilizes small sample size (10 μL/well*)
- Quantitative
- Validated for cell culture supernates, serum, plasma, saliva, and urine
- 4.5 hour assay time
- Amenable to automation
*All sample types require a dilution.
Kit Contents**
- 96-well microplate with each well spotted with 7 capture antibodies & 1 reference spot
- Cocktail of purified recombinant human MMPs
- Cocktail of biotinylated detection antibodies
- Streptavidin-HRP
- Diluents & Wash Buffers
- Chemiluminescent Reagents***
- Plate Covers
**Acquisition of data from Mosaic ELISAs requires a Digital Imaging System.
***Substrates 1 and 2 are comprised of TMA-6, a product of Lumigen, Inc., Southfield, Michigan, USA, and are covered by the following US Patent Numbers: 5,922,558 and 6,858,733 and International Patent Numbers: 733,086, 1,019,525, 2,300,071, 1,015,461, 2,002,352,881, ZL02805225.0, and 1,456,716