Product Category
Mosaic™ ELISA Human Cytokine Panel 1
The Mosaic ELISA Human Cytokine Panel 1 (Catalog # MEA001) is an excellent tool for the simultaneous quantitative determination of 8 different cytokines in a single sample of cell culture supernate, serum, and plasma. Mosaic ELISA kits employ multiplex microarray technology to provide an accurate, efficient, and economical alternative to conducting multiple traditional ELISA experiments.
| Antibody Well Map |
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| A visualization of the spot layout per well in the Mosaic ELISA Human Cytokine Panel 1 (Catalog # MEA001). Each individual well is spotted with 8 different antibodies and a Reference Spot. Reference Spots provide a strong positive signal to allow easy visualization of well location for template alignment during data analysis. |
Features
- Detects 8 cytokines in a single sample
- An array in each well of a 96-well microplate
- 320 data points per microplate (40 samples in duplicate plus 2 standard curves)
- Utilizes small sample size (25 μL/well*)
- Quantitative
- Validated for cell culture supernates, serum, and plasma
- 4.5 hour assay time
- Amenable to automation
*Serum/plasma/platelet-poor plasma samples require a 2-fold dilution.
Kit Contents**
- 96-well microplate with each well spotted with 8 capture antibodies & 1 reference spot
- Cocktail of purified recombinant human cytokines
- Cocktail of biotinylated detection antibodies
- Streptavidin-HRP
- Diluents & Wash Buffers
- Chemiluminescent Reagents***
- Plate Covers
**Acquisition of data from Mosaic ELISAs requires a Digital Imaging System.
***Substrates 1 and 2 are comprised of TMA-6, a product of Lumigen, Inc., Southfield, Michigan, USA, and are covered by the following US Patent Numbers: 5,922,558 and 6,858,733 and International Patent Numbers: 733,086, 1,019,525, 2,300,071, 1,015,461, 2,002,352,881, ZL02805225.0, and 1,456,716