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• Mouse Phospho-RTK Array Kit Ordering Information

Introduction

R&D Systems' Mouse Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY014) is a screening tool designed to simultaneously detect the relative phosphorylation of 39 different RTKs. The Kit eliminates the need for numerous immunoprecipitation (IP) and/or Western blot experiments. RTKs are captured by antibodies spotted on a nitrocellulose membrane. Levels of phospho-RTK are then assessed using an HRP-conjugated pan phospho-tyrosine antibody followed by chemiluminescence detection.

• Product Insert
• Troubleshooting Guide
• Assays for analytes represented in to the Mouse Phospho-Receptor Tyrosine Kinase Array Kit
• Western blotting antibodies for analytes related to the Mouse Phospho-Receptor Tyrosine Kinase Array Kit

Simultaneously detect the relative phosphorylation of these RTKs in a single sample
EGF R	PDGF R alpha	VEGF R3
ErbB2	PDGF R beta	MuSK
ErbB3	SCF R	EphA1
ErbB4	Flt-3	EphA2
FGF R2 (IIIc)	M-CSF R	EphA3
FGF R3	c-Ret	EphA6
FGF R4	Tie-1	EphA7
Insulin R	Tie-2	EphA8
IGF-I R	TrkA	EphB1
Axl	TrkB	EphB2
DtK	TrkC	EphB4
Mer	VEGF R1	EphB6
HGF R	VEGF R2
MSP R

Product Features

• SPECIFIC - Carefully selected panel of 39 capture antibodies gives low cross-reactivity
• EFFICIENT - Tests 39 different RTKs simultaneously with a small sample size
• SENSITIVE - More sensitive than IP-Western blots
• CONVENIENT - Utilizes chemiluminescence detection
• FLEXIBLE - Protocol may be tailored to specific needs

Kit Contents

Each Mouse Phospho-RTK Array Kit contains most of the necessary components for a ready-to-run assay without the need for specialized equipment.*

• 4-Single Use Nitrocellulose Membranes spotted in duplicate with capture and control antibodies
• 4-Well Multi-dish
• Lysis Buffer 15 (for cell lysis)
• Array Buffer 1 (for blocking and sample incubation)
• Array Buffer 2 Concentrate, 5X (for dilution of the detection antibody)
• Wash Buffer Concentrate, 25X (for washing the arrays after lysate and detection antibody incubations)
• Anti-Phospho-Tyrosine-HRP Detection Antibody
• Transparency Overlay Template
• Detailed product insert with protocol

*Chemiluminescent detection substrate not included. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product insert.