Introduction
| Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. |
| CXCL13/BLC/BCA-1 |
IL-16 |
| C5a |
IL-17 |
| G-CSF |
IL-23 |
| GM-CSF |
IL-27 |
| CCL1/I-309 |
CXCL10/IP-10 |
| CCL11/Eotaxin |
CXCL11/I-TAC |
| ICAM-1 |
KC |
| IFN-gamma |
M-CSF |
| IL-1 alpha |
CCL2/JE/MCP-1 |
| IL-1 beta |
CCL12/MCP-5 |
| IL-1ra |
CXCL9/MIG |
| IL-2 |
CCL3/MIP-1 alpha |
| IL-3 |
CCL4/MIP-1 beta |
| IL-4 |
MIP-2 |
| IL-5 |
CCL5/RANTES |
| IL-6 |
CXCL12/SDF-1 |
| IL-7 |
CCL17/TARC |
| IL-10 |
TIMP-1 |
| IL-12 p70 |
TNF-alpha |
| IL-13 |
TREM-1 |
R&D Systems Mouse Cytokine Array, Panel A (Catalog # ARY006) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 40 different cytokines, chemokines, and more in a single sample. No specialized equipment is necessary
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, serum, or plasma samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents* are added and a signal is produced in proportion to the amount of cytokine bound.
Kit Contents
- 4 Array Membranes
- Array Buffers
- Wash Buffer
- Antibody Detection Cocktail
- Streptavidin-HRP
- Transparency Overlay Template
- Detailed Protocol
*Chemiluminescence detection substrate not included. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product insert ( PDF 116 KB).