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Mouse Adipokine Antibody Array

Introduction

R&D Systems Mouse Adipokine Array (Catalog # ARY013) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 38 different obesity-related molecules in a single sample. No specialized equipment is necessary

Simultaneously detect the levels of these obesity-related proteins in a single sample.
Adiponectin IGF-I M-CSF
AgRP IGF-II OSM
ANGPT-L3 IGFBP-1 PAI-1/Serpin E1
CCL2/MCP-1/JE IGFBP-2 PTX2
CCL5/RANTES IGFBP-3 PTX3
CRP IGFBP-5 Pref-1
DPPIV/CD26 IGFBP-6 RAGE
Endocan IL-6 RBP4
Fetuin A IL-10 Resistin
FGF acidic IL-11 TIMP-1
FGF-21 Leptin TNF-alpha
HGF LIF VEGF
ICAM-1 Lipocalin-2/NGAL

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, or serum samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

Kit Contents

  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product datasheet.