Product Category
Mouse Adipokine Antibody Array
Introduction
R&D Systems Mouse Adipokine Array (Catalog # ARY013) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 38 different obesity-related molecules in a single sample. No specialized equipment is necessary
| Simultaneously detect the levels of these obesity-related proteins in a single sample. |
| Adiponectin |
IGF-I |
M-CSF |
| AgRP |
IGF-II |
OSM |
| ANGPT-L3 |
IGFBP-1 |
PAI-1/Serpin E1 |
| CCL2/MCP-1/JE |
IGFBP-2 |
PTX2 |
| CCL5/RANTES |
IGFBP-3 |
PTX3 |
| CRP |
IGFBP-5 |
Pref-1 |
| DPPIV/CD26 |
IGFBP-6 |
RAGE |
| Endocan |
IL-6 |
RBP4 |
| Fetuin A |
IL-10 |
Resistin |
| FGF acidic |
IL-11 |
TIMP-1 |
| FGF-21 |
Leptin |
TNF-alpha |
| HGF |
LIF |
VEGF |
| ICAM-1 |
Lipocalin-2/NGAL |
|
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, serum, or plasma samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents* are added and a signal is produced in proportion to the amount of cytokine bound.
Kit Contents
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Antibody Detection Cocktail
- Streptavidin-HRP
- Transparency Overlay Template
- Detailed Protocol
*Chemiluminescence detection substrate not included. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product insert.