With Fluorokine® MultiAnalyte Profiling (MAP) and the Luminex® 200™ analyzer, you can measure multiple analytes in a single 50 microliter aliquot of serum, plasma, or cell culture supernatant.* Microparticle beads are dyed with differing concentrations of two fluorophores to generate distinct bead sets. Each bead set is coated with capture antibody specific for one analyte. Captured analyte is detected using a biotinylated detection antibody and streptavidin-phycoerythrin (S-PE). The Luminex 200 analyzer is a dual laser, flow-based, sorting and detection platform. One laser is bead-specific and determines which cytokine is being detected. The other laser determines the magnitude of PE-derived signal, which is in direct proportion to the amount of analyte bound.
* Sample volumes may be smaller, please see specific package inserts for details.
Quality & Performance
R&D Systems Fluorokine MAP kits are subject to the same development, validation, manufacturing, and quality control standards as our Quantikine® ELISAs, the world's most referenced cytokine immunoassays. The result is the most accurate, sensitive, and reproducible cytokine multiplex assay available. For each sample type (serum, plasma, and cell culture supernates), Fluorokine MAP is evaluated and validated on the following performance characteristics: sensitivity, precision, recovery, sample linearity, specificity, and the ability to recognize both the natural and recombinant analyte. Further, all analytes are tested together as a panel to ensure multiplex compatibility.
Luminex® 100™ and 200™ analyzers, software, and service packages are available through the following preferred vendors. Several companies offer modified Luminex analyzers such as the Bio-Plex® (Bio-Rad Laboratories, Inc.) and LiquiChip® (Qiagen). For information on running Fluorokine MAP kits on the LiquiChip or Bio-Plex analyzers consult the operating manual to change the template for the Fluorokine MAP bead regions. If you have any questions please call our technical service department at (800) 343-7475, or fill out our Contact Us form.
Applied Cytometry Inc.
101 E Park Blvd. Suite 600
Plano, TX 75074
Tel (866) 500-FLOW
601 Gateway Blvd. Suite 100
South San Fransisco, CA 94080
Tel (800) 624-6176
Luminex is a registered trademark of Luminex Corporation
Luminex 100 is a trademark of Luminex Corporation
Luminex 200 is a trademark of Luminex Corporation
LiquiChip is a registered trademark of Qiagen
Bio-Plex is a registered trademark of Bio-Rad Laboratories, Inc.