• Complete listing of PlusCellect™ products

R&D Systems PlusCellect cell isolation kits supply two antibodies, one for immunopurification and the other for immunophenotypical analysis. Cell isolation is done by positive selection. Cells of interest are tagged with a biotinylated antibody followed by streptavidin-conjugated magnetic particles (MagCellect™ Streptavidin Ferrofluid, Catalog # MAG999 or equivalent). Tagged cells can be isolated using any streptavidin-ferrofluid/magnet system. Once the cells are resuspended in PlusCellect Buffer, the efficiency of enrichment can be assessed with the provided PE-conjugated detection antibody.

Assay Principle

Kit Components

• Biotinylated analyte specific Selection Antibody
• PE-conjugated Detection Antibody
• PlusCellect Buffer

Advantages

• Rapid
• Specific
• Two analyte-specific antibodies recognizing different epitopes are supplied
• May be used with any streptavidin-ferrofluid and magnet system
• Unique target molecules
• Flexible format
• May be used in conjunction with negative selection prior to the positive selection step for even greater purity of small cell populations.
• Both negative and positive cell fractions are still viable after separation

Isolation of human EpCAM+ cells. Whole blood cells were spiked with EpCAM-expressing MCF-7 human breast cancer cells. Cells were stained with the provided PE-labeled EpCAM detection antibody both before and after enrichment for EpCAM+ cells using the human EpCAM PlusCellect Kit (Catalog # PLS960).

PlusCellect Antigen Expression