Cell Selection Kits| PlusCellect™ Kits
PlusCellect™ Kits

R&D Systems PlusCellect cell isolation kits supply two antibodies, one for immunopurification and the other for immunophenotypical analysis. Cell isolation is done by positive selection. Cells of interest are tagged with a biotinylated antibody followed by streptavidin-conjugated magnetic particles (MagCellectâ„¢ Streptavidin Ferrofluid, Catalog # MAG999 or equivalent). Tagged cells can be isolated using any streptavidin-ferrofluid/magnet system. Once the cells are resuspended in PlusCellect Buffer, the efficiency of enrichment can be assessed with the provided PE-conjugated detection antibody.

Assay Principle

Kit Components

  • Biotinylated analyte specific Selection Antibody
  • PE-conjugated Detection Antibody
  • PlusCellect Buffer

Advantages

  • Rapid
  • Specific
  • Two analyte-specific antibodies recognizing different epitopes are supplied
  • May be used with any streptavidin-ferrofluid and magnet system
  • Unique target molecules
  • Flexible format
  • May be used in conjunction with negative selection prior to the positive selection step for even greater purity of small cell populations.
  • Both negative and positive cell fractions are still viable after separation
PlusCellect Sample Data
Isolation of human EpCAM+ cells. Whole blood cells were spiked with EpCAM-expressing MCF-7 human breast cancer cells. Cells were stained with the provided PE-labeled EpCAM detection antibody both before and after enrichment for EpCAM+ cells using the human EpCAM PlusCellect Kit (Catalog # PLS960).

PlusCellect Antigen Expression

Analyte Catalog #

Cell Types (*)

TC BC DC NK SC Mo Gr Pl Er EC Ep
VEGF R2 PLS357 + +
Integrin alpha V/
CD51
PLS1219 + - + +
Endoglin/
CD105
PLS1097 - - - + + - - - +
ACE/
CD143
PLS929 - - - + - - + +
VE-Cadherin PLS938 - - - - - - - - + -
MCAM/
CD146
PLS932 + - - - - - - +
CD31/
PECAM-1
PLS806 + + + + + + - +
EpCAM PLS960 +
E-Cadherin PLS1838 + + +
CXCR4 PLS170 + + + + +
CCR5 PLS180 + - - + + - - -
CD4 PLS379 + - - - + + - - - -
NCAM-1/
CD56
PLS2408 +
CD45 PLS1430 + + + + + + + - - - -
VCAM-1/CD106 PLS809                   +  
E-Selectin/CD62E PLS724                   +  
(*) TC=T-cells, BC=B-cells, DC=Dendritic Cells, NK=Natural Killer cells, SC=Stem Cells, Mo=Monocytes, Gr=Granulocytes, Pl=Platelets, Er=Erythrocytes, EC=Endothelial Cells, Ep=Epithelial Cells




RELATED INFORMATION
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