• PRODUCTS
•  
• TECHNICAL INFORMATION
•  
• CUSTOMER SERVICE
•  
• ABOUT US
•  
• HEMATOLOGY
•  

The Proteome Profiler 96 Human Phospho-RTK Array 1, Infrared (Catalog # ARZ004) ) employs a two-site sandwich immunoassay technique to simultaneously detect 7 phosphorylated kinases in a single sample of cell lysate. This assay also detects HSP60, which serves as an internal control. Multiple capture antibodies that specifically recognize the target analytes detected by the assay have been spotted into each well of a 96-well microplate.

Antibody Well Map

A visualization of the spot layout per well in the Proteome Profiler 96 Phospho-Kinase Array 1 (Catalog # ARZ004).  Each individual well is spotted with 7 different antibodies and 1 Reference Spot.  The Reference Spot provides a strong positive signal to allow easy visualization of well location for template alignment during data analysis.

Features

• Predefined microplate-based multiplex assay
• Simultaneously detects 7 phosphorylated kinases
• Loading control in each well
• Utilizes small sample size (5-50 μg of cell lysate)
• 3 hour assay time
• Amenable to high-throughput
• Chemiluminescence detection

Kit Contents

• 96-well microplate with each well spotted with 8 capture antibodies & 1 reference spot
• Detection Antibody Cocktail
• Streptavidin-HRP
• Array & Wash Buffers
• Cell Lysis Buffer
• Substrate Reagents**
• Plate Covers

*Acquisition of data from Proteome Profiler 96 Microplate-based Antibody Arrays requires a digital imaging systems.

**Substrates 1 and 2 are comprised of TMA-6, a product of Lumigen, Inc., Southfield, Michigan, USA, and are covered by the following US Patent Numbers: 5,922,558 and 6,858,733; and International Patent Numbers: 733,086, 1,019,525, 2,300,071, 1,015,461, 2,002,352,881, ZL02805225.0, and 1,456,716

Data Example

UV Light Induces Phosphorylation of Intracellular Kinases in HeLa Cells

A. The Proteome Profiler 96 Human Phospho-Kinase Antibody Array 1 (Catalog # ARZ004) was used to simultaneously assess the phosphorylation of 7 different intracellular kinases in lysates from the HeLa human cervical epithelial carcinoma cell line either untreated or exposed to 15 mJ UV light followed by a 30 minute recovery period. RS=Reference Spot. B. Histogram profiles for select kinases were generated by quantifying the mean spot pixel densities from individual antibody spots using analytical software.