Product Category
Rat Adipokine Antibody Array
Introduction
R&D Systems Rat Adipokine Array (Catalog # ARY016) is a rapid, sensitive, and economical tool used to simultaneously detect the relative levels of 30 different obesity-related molecules in a single sample. No specialized equipment is necessary.
| Simultaneously detect the levels of these obesity-related proteins in a single sample. |
| ANGPT-L3 |
IGFBP-1 |
LIF |
| CCL2/MCP-1/JE |
IGFBP-2 |
Lipocalin-2/NGAL |
| CCL5/RANTES |
IGFBP-3 |
M-CSF |
| DPPIV/CD26 |
IGFBP-5 |
PAI-1/Serpin E1 |
| Endocan |
IGFBP-6 |
Pref-1 |
| FGF-21 |
IL-1 beta |
RAGE |
| HGF |
IL-6 |
Resistin |
| ICAM-1 |
IL-10 |
TIMP-1 |
| IGF-I |
IL-11 |
TNF-alpha |
| IGF-II |
Leptin |
VEGF |
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell and tissue lysate, serum, or plasma samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents* are added and a signal is produced in proportion to the amount of cytokine bound.
Kit Contents
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Antibody Detection Cocktail
- Streptavidin-HRP
- Transparency Overlay Template
- Detailed Protocol
*Chemiluminescence detection substrate not included. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product insert.