Introduction
R&D Systems Rat Cytokine Array (Catalog # ARY008) is a rapid, sensitive, and economical tool to simultaneously detect the relative expression of 29 cytokine and chemokines in a single sample. No specialized equipment is necessary.
| Simultaneously detect the levels of these cytokines in a single sample. |
| CXCL1/CINC-1 |
IL-1ra/IL-1F3 |
L-Selectin |
| CXCL3/CINC-2 alpha/beta |
IL-2 |
CXCL9/MIG |
| CINC-3 |
IL-3 |
CCL3/MIP-1 alpha |
| CNTF |
IL-4 |
CCL20/MIP-3 alpha |
| Fractalkine/CXC3CL1 |
IL-6 |
CCL5/RANTES |
| GM-CSF |
IL-10 |
Thymus Chemokine |
| ICAM-1 |
IL-13 |
TIMP-1 |
| IFN-gamma |
IL-17 |
TNF-alpha/TNFSF1A |
| IL-1 alpha/IL-1F1 |
CXCL10/IP-10 |
VEGF |
| IL-1 beta/IL-1F2 |
LIX |
|
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, serum, or plasma samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents* are added and a signal is produced in proportion to the amount of cytokine bound.
Kit Contents
- 4 Array Membranes
- Array Buffers
- Wash Buffer
- Antibody Detection Cocktail
- Streptavidin-HRP
- Transparency Overlay Template
- Detailed Protocol
*Chemiluminescence detection substrate not included. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product insert ( PDF 186K).