Proteome Profiler Antibody Arrays| Human Phospho-Receptor Tyrosine Kinase Array Kit

• Human Phospho-RTK Array Kit Ordering Information

Introduction

R&D Systems' Human Phospho-Receptor Tyrosine Kinase (RTK) Array Kit (Catalog # ARY001) is a screening tool designed to simultaneously detect the relative phosphorylation of 42 different RTKs. The Kit eliminates the need for numerous immunoprecipitation (IP) and/or Western blot experiments. RTKs are captured by antibodies spotted on a nitrocellulose membrane. Levels of phospho-RTK are then assessed using an HRP-conjugated pan phospho-tyrosine antibody followed by chemiluminescence detection.

• Product Insert (PDF, 116K)
• Troubleshooting Guide
• Assays for analytes represented in the Human Phospho-Receptor Tyrosine Kinase Array Kit
• Western blotting antibodies for analytes represented in the Human Phospho-Receptor Tyrosine Kinase Array Kit

Simultaneously detect the relative phosphorylation of these RTKs in a single sample
Axl	ErbB3	PDGF R alpha
Dtk	ErbB4	PDGF R beta
EGF R	FGF R1	c-Ret
EphA1	FGF R2 alpha	ROR1
EphA2	FGF R3	ROR2
EphA3	FGF R4	SCF R
EphA4	Flt-3	Tie-1
EphA6	HGF R	Tie-2
EphA7	IGF-I R	TrkA
EphB1	Insulin R	TrkB
EphB2	M-CSF R	TrkC
EphB4	Mer	VEGF R1
EphB6	MSP R	VEGF R2
ErbB2	MuSK	VEGF R3

Product Features

• SPECIFIC - Carefully selected panel of 42 capture antibodies gives low cross-reactivity
• RAPID - 5 hour assay time
• EFFICIENT - Tests 42 different RTKs simultaneously with a small sample size
• SENSITIVE - Up to 20 times more sensitive than IP-Western blots
• CONVENIENT - Utilizes chemiluminescence detection
• FLEXIBLE - Protocol may be tailored to specific needs

Kit Contents

Each Human Phospho-RTK Array Kit contains most of the necessary components for a ready-to-run assay without the need for specialized equipment.*

• 4-Single Use Nitrocellulose Membranes spotted in duplicate with capture and control antibodies
• 4-Well Multi-dish
• Array Buffer 1 (for blocking and sample incubation)
• Array Buffer 2 Concentrate, 5X (for dilution of the detection antibody)
• Wash Buffer Concentrate, 25X (for washing the arrays after lysate and detection antibody incubations)
• Anti-Phospho-Tyrosine-HRP Detection Antibody
• Transparency Overlay Template
• Detailed product insert with protocol

*Chemiluminescent detection substrate not included. For a complete list of the kit contents and necessary materials, please see the Materials Provided/Materials Required sections of the product insert (PDF, 316K).