Product Category

Single Analyte ELISpot Kits

R&D Systems' ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Complete ELISpot kits are ready-to-run and require no assay development or refinement.

ELISpot assays are used to measure responses with frequencies well below 1 in 100,000 cells. This technique does not require prior in vitro expansion of cells and is suitable for high-throughput analysis using only small numbers of primary cells. As such, ELISpot assays are useful tools for research in vaccine development.

Assay Principle
View Full Assay Principle

Features

  • Detect and quantitate individual cells secreting specific analytes
  • High sensitivity - ELISpot assays can measure responses with frequencies well below 1 in 100,000 cells
  • No in vitro expansion of cells required
  • High-throughput - ELISpot assays use only a small number of primary cells

Single Color Kit Contents

  • Coated PVDF-backed 96-well Microplate
  • Detection Antibody
  • Streptavidin-AP
  • BCIP/NBT
  • Positive Control
  • Dilution Buffers
  • Wash Buffer