• Complete Listing of Cell Enrichment Column Kits & Reagents

Cell Enrichment Columns are available to purify all T cells and specific T cell subpopulations by high affinity negative selection.

T Cell Enrichment Columns

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Mononuclear cell suspensions are loaded onto T Cell Enrichment Columns containing glass beads coated with Ig and anti-Ig. B cells bind to anti-Ig coated beads by F(ab)-surface Immunoglobulin (Ig) interactions. Monocytes bind to Ig coated beads via Fc interactions. The resulting column eluate contains highly enriched T cell populations.

CD3⁺ is a universal marker for T cells. Total CD3⁺ cell recovery ranges between 30% and 50% of initial CD3⁺ cells loaded. The purity of recovered cells ranges from 85% to 95%. These enriched T cell populations are then available for cell culture, activation studies, phenotyping, immune status monitoring, and flow cytometry.

Features

• Available for human, mouse, and rat cells
• Purifies CD3⁺ T cells to 85 – 95%
• Based upon gravitational flow
• Simple procedure
• No specialized equipment required

Kit Contents

• Cell Selection Column
• 10X Column Buffer

Subtype Specific T Cell Enrichment Columns

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T Cell Enrichment Column Kits are also available to isolate specific T cell subpopulations of interest. Before loading onto T Cell Subset Columns, leukocyte suspensions are incubated with a mixture of monoclonal antibodies. Monoclonal antibody cocktails are designed to recognize undesired T cells and other undesired cell populations. Samples are subsequently loaded onto enrichment columns containing glass beads coated with Ig and anti-Ig. Following simple gravitational flow, B cells, monocytes, and the undesired T cells are removed from the sample via high affinity negative selection. Kits are available for the purification of CD4⁺, CD8⁺, memory, and naïve T cells.

Features

• Available for specific CD4⁺/CD8⁺ T cell populations
• Purifies desired T cell population to 85 – 95%
• Minimal depletion of desired T cell type

Kit Contents

• Monoclonal Antibody Cocktail
• Cell Selection Column
• 10X Column Buffer

Kits Available For:

A	B
Figure 1. (A) A CD3-FITC and CD8-PE stained mouse mononuclear cell preparation prior to loading on a mouse CD4+ subset enrichment column. (B) A CD3-FITC and CD8-PE stained sample following application to a mouse CD4+ enrichment selection column. Note that all of the CD3+/CD8+ T cells have been depleted from the mouse mononuclear cell preparation.