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HIV-1 Gag p24 Quantikine ELISA Kit

Catalog #: DHP240
11 Citations Datasheet / COA / SDS
Newer Version Available: DHP240B
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Discontinued Product

DHP240 has been discontinued and is replaced by DHP240B.

R&D Systems Quantikine ELISAs
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Citations (11)
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Summary Recovery Linearity Product Datasheets Preparation and Storage Background Related Research Areas

HIV-1 Gag p24 Quantikine ELISA Kit Summary

Assay Length
80 minutes
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
3.25 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human CD14.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® QuicKit™ Human CD14 Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human CD14 levels in cell culture supernates, serum, and plasma. It contains CHO-expressed recombinant human CD14 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human CD14 showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit™ standards. These results indicate that this kit can be used determine relative mass values for natural CD14.

Recovery

The recovery of human CD14 spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 100 93-105
EDTA Plasma (n=4) 74 64-82
EDTA Plasma (ICD Treated) (n=4) 88 64-94
Heparin Plasma (n=4) 72 63-78
Heparin Plasma (ICD Treated) (n=4) 88 69-94
Serum (n=4) 79 73-85

Linearity

To assess the linearity of the assay, samples containing high concentrations of human CD14 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. 

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: HIV-1 Gag p24

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Long Name:
Human Immunodeficiency Virus Type 1 GAG/Group-specific Antigen core protein of 24 kDa
Entrez Gene IDs:
155030 (Viral)
Alternate Names:
CA; Capsid Protein; HIV1 Gag p24; HIV-1 Gag p24; Pr55(Gag)
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for HIV-1 Gag p24 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Serological study of CoronaVac vaccine and booster doses in Chile: immunogenicity and persistence of anti-SARS-CoV-2 spike antibodies
    Authors: L Vargas, N Valdivieso, F Tempio, V Simon, D Sauma, L Valenzuela, C Beltrán, L Castillo-D, X Contreras-, ML Acevedo, J Acevedo, RI Gonzalez, F Valiente-E, R Soto-Rifo, M Rosemblatt, M Lopez, F Osorio, MR Bono
    Bmc Medicine, 2022-06-09;20(1):216.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Differential neutralizing antibody responses elicited by CoronaVac and BNT162b2 against SARS-CoV-2 Lambda in Chile
    Authors: ML Acevedo, A Gaete-Arge, L Alonso-Pal, MM de Oca, A Bustamante, A Gaggero, F Paredes, CP Cortes, S Pantano, C Martínez-V, J Angulo, N Le Corre, M Ferrés, MA Navarrete, F Valiente-E, R Soto-Rifo
    Nature Microbiology, 2022-04-01;7(4):524-529.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Epitranscriptomic regulation of HIV-1 full-length RNA packaging
    Authors: C Pereira-Mo, D Toro-Ascuy, C Ananías-Sá, A Gaete-Arge, C Rojas-Fuen, S Riquelme-B, B Rojas-Aray, F García-de-, P Aguilera-C, J Chnaiderma, ML Acevedo, F Valiente-E, R Soto-Rifo
    Nucleic Acids Research, 2022-02-28;0(0):.
    Species: Human
    Sample Types: Whole Cells
  4. Multiple expansions of globally uncommon SARS-CoV-2 lineages in Nigeria
    Authors: EA Ozer, LM Simons, OM Adewumi, AA Fowotade, EC Omoruyi, JA Adeniji, OA Olayinka, TJ Dean, J Zayas, PP Bhimalli, MK Ash, AI Maiga, AM Somboro, M Maiga, A Godzik, JR Schneider, JI Mamede, BO Taiwo, JF Hultquist, R Lorenzo-Re
    Nature Communications, 2022-02-03;13(1):688.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. CCR5 antagonist reduces HIV-induced amyloidogenesis, tau pathology, neurodegeneration, and blood-brain barrier alterations in HIV-infected hu-PBL-NSG mice
    Authors: B Bhargavan, SM Woollard, JE McMillan, GD Kanmogne
    Molecular Neurodegeneration, 2021-11-22;16(1):78.
    Species: Mouse
    Sample Types: Plasma
  6. High prevalence of SARS-CoV-2 B.1.1.7 (UK variant) and the novel B.1.5.2.5 lineage in Oyo State, Nigeria
    Authors: EA Ozer, LM Simons, OM Adewumi, AA Fowotade, EC Omoruyi, JA Adeniji, TJ Dean, J Zayas, PP Bhimalli, MK Ash, A Godzik, JR Schneider, JI Mamede, BO Taiwo, JF Hultquist, R Lorenzo-Re
    medRxiv : the preprint server for health sciences, 2021-07-02;0(0):.
    Species: Human
    Sample Types: Virus
  7. Plasmacytoid dendritic cells have divergent effects on HIV infection of initial target cells and induce a pro-retention phenotype
    Authors: O Tong, G Duette, TR O'Neil, CM Royle, H Rana, B Johnson, N Popovic, S Dervish, MAE Brouwer, H Baharlou, E Patrick, G Ctercteko, S Palmer, E Lee, E Hunter, AN Harman, AL Cunningham, N Nasr
    PloS Pathogens, 2021-04-19;17(4):e1009522.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. A New Generation of Functional Tagged Proteins for HIV Fluorescence Imaging
    Authors: JI Mamede, J Griffin, S Gambut, TJ Hope
    Viruses, 2021-02-28;13(3):.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Insights into neutralizing antibody responses in individuals exposed to SARS-CoV-2 in Chile
    Authors: C Beltrán-Pa, S Riquelme-B, A Oyarzún-Ar, A Gaete-Arge, R González-S, K Cereceda-S, A Aguirre, D Travisany, R Palma-Veja, GP Barriga, A Gaggero, C Martínez-V, NL Corre, M Ferrés, ME Balcells, J Fernandez, E Ramírez, F Villarroel, F Valiente-E, R Soto-Rifo
    Science Advances, 2021-02-12;7(7):.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Epigenetics, N-myrystoyltransferase-1 and casein kinase-2-alpha modulates the increased replication of HIV-1 CRF02_AG, compared to subtype-B viruses
    Authors: B Bhargavan, GD Kanmogne
    Sci Rep, 2019-07-23;9(1):10689.
    Species: Human
    Sample Types: Plasma
  11. Automation of human pluripotent stem cell differentiation toward retinal pigment epithelial cells for large-scale productions
    Authors: F Regent, L Morizur, L Lesueur, W Habeler, A Plancheron, K Ben M'Bare, C Monville
    Sci Rep, 2019-07-23;9(1):10646.
    Species: Human
    Sample Types: Plasma

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