Weekly New Products


    New Products September 07, 2010

    Featured Product: Human/Mouse Total COX-2 Cell-Based ELISA

    Analysis of IL-1beta-induced COX-2 Expression using the COX-2 Cell-Based ELISA. CCD-1070SK human foreskin fibroblasts were cultured in 96-well microplates and treated with the indicated concentrations of Recombinant Human IL-1 beta (Catalog # 201-LB) for 24 hours. COX-2 levels were determined using the Human/Mouse Total COX-2 Cell-Based ELISA (Catalog # KCB4198) and normalized to total GAPDH detected in the same microplate wells (bar graph). Western blot analysis using the antibodies provided in the kit is shown for comparison (inset).

    R&D Systems now offers a Human/Mouse Total COX-2 Cell-Based ELISA (Catalog # KCB4198). This fluorescence-based assay simultaneously measures COX-2 and GAPDH in the same microplate well without the need for lysate preparation. Normalization of the fluorescent signal derived from COX-2 to that of the GAPDH protein corrects for well-to-well variations such as differences in cell number. This Cell-Based ELISA Kit includes primary antibodies to COX-2 and GAPDH, species-specific secondary antibodies conjugated to either horseradish peroxidase (HRP) or alkaline phosphatase (AP), spectrally distinct fluorogenic substrates for each, and wash and blocking buffers. Cell-Based ELISAs require no specialized equipment and can be run with as few as 10,000 cells per well, making them an excellent alternative to Western blot. For more information and a complete product listing, please visit our website at www.RnDSystems.com/go/CellBasedELISA or call 1-800-343-7475.

     

    MOLECULEDESCRIPTION
    CATALOG #/
    DATASHEET
    SIZE
     
    Antibodies
    CD55/DAF Mouse CD55/DAF Phycoerythrin MAb (Clone 583905) FAB5376P100 Tests
    TGF‑beta RII Mouse TGF-beta RII Fluorescein Affinity Purified PAb FAB532F100 Tests
    ILT7/CD85g Human ILT7/CD85g Allophycocyanin MAb (Clone 656688) FAB6287A100 Tests
    ILT7/CD85g Human ILT7/CD85g Phycoerythrin MAb (Clone 656688) FAB6287P100 Tests
    ILT7/CD85g Human ILT7/CD85g Fluorescein MAb (Clone 656688) FAB6287F100 Tests
    ILT7/CD85g Human ILT7/CD85g PerCP MAb (Clone 656688) FAB6287C100 Tests
    BDNF Human Pro-BDNF MAb (Clone 584421) MAB3175100 µg
    CD55/DAF Mouse CD55/DAF MAb (Clone 583905) MAB5376100 µg
    CEACAM‑4 Human CEACAM-4 MAb (Clone 568410) MAB4354100 µg
    Carbohydrate Sulfotransferase 1/CHST1 Human CHST1 MAb (Clone 543128) MAB5316100 µg
    PAR/pADPr PAR Affinity Purified Polyclonal Ab 4336-APC-05050 µL
    Fc gamma RII/CD32 Human Fc gamma RII/CD32 Allophycocyanin MAb (Clone 190723) FAB1330A100 Tests
    DLEC/CLEC4C/BDCA‑2 Human DLEC/CLEC4C/BDCA-2 Phycoerythrin MAb (Clone 628817) FAB6299P100 Tests
    Nectin‑1 Human Nectin-1 Allophycocyanin MAb (Clone 610835) FAB2880A100 Tests
    IFN‑alpha/beta R2 Mouse IFN-alpha/beta R2 Fluorescein Affinity Purified PAb FAB1083F100 Tests
    Cyclin D1 Human Cyclin D1 PerCP MAb (Clone 608030) IC43141C100 Tests
    LRP-1 Human LRP-1 MAb (Clone 545503) MAB6360100 µg
     
    ELISAs
    COX‑2 Human/Mouse Total COX-2 Cell-Based ELISA KCB41981 Kit
    COX‑2 Human/Mouse Total COX-2 Cell-Based ELISA KCB41981 Kit
     
    Recombinant & Natural Proteins
    ROBO4 Recombinant Human ROBO4/Fc Chimera, CF 2366-RB-05050 µg


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