Troubleshooting Guide: MycoProbe® Mycoplasma Detection Kit

Observation: High background level

Problem	Corrective Action
Insufficient washing	Wash per protocol being sure to remove all Wash Buffer from wells before addition of next component
Contamination with alkaline phosphatase	Keep work area clean and free of alkaline phosphatase

Observation: Poor precision

Problem	Corrective Action
Plate not washed before use	Wash per protocol
RNase contamination	Use RNase-free technique
Pipetting error	Use a new pipet tip for each pipetting step and use proper technique

Observation: No signal for positive control

Problem	Corrective Action
Component or step omitted	Read protocol thoroughly before repeating the assay
RNase contamination	Use RNase-free technique