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Troubleshooting Guide: Proteome Purify™ Immunodepletion Kits
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Problem: Incomplete or no flow through spin filter
| Cause |
Solution |
| Cap on too tight |
Loosen or remove spin filter cap. |
| Air lock |
Tap side of spin filter to dislodge air
bubble |
Centrifuge parameters need to be adjusted |
2 minutes at 1000-2000 x g usually sufficient. |
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