The CometChip® platform is a high-throughput single-cell gel electrophoresis version of the CometAssay®, a sensitive technique used to detect and measure DNA damage and repair in various cell types. The variation between studies in the slide based CometAssay has an adverse impact on reproducibility. The CometChip is able to overcome these reproducibility issues with uniformly dispersed micropores in the gel. Each micropore traps one cell in a single focal plane and each well in the 96-well CometChip contains 400 microwells, allowing measurement of up to 38,400 comets per chip. The CometAssay Analysis Software has automated comet acquisition and scoring that can analyze more than half a million comets per week using the CometAssay Electrophoresis System.
Learn more about R&D Systems CometAssay and CometChip
We demonstrate how CometChip's versatility can be used with different in vitro models such as HepaRG™ cells, 2D cell culture of induced pluripotent stem cell (iPSC)-derived hepatocyte-like cells, and liver organoids. Variability between the CometChip assays are less than 10%, with no overlapping comets requiring manual editing. The well-to-well CVs are consistent with US Pharmacopeia (USP) guidelines for 96-well assays.
Additional CometAssay Resources
Choosing the Right Model System for Hepatotoxicity
DNA Damage and Repair Kits & Reagents
CometAssay System Brochure
CometAssay Analysis Software Brochure
CometAssay Assay Principle