Expanding Cryopreserved Epithelial-derived Adult Stem Cells

MimEX^™ Tissue Models System provides a platform for the isolation, expansion, and differentiation of adult epithelial stem cells into 3-D ex vivo organ tissue. This protocol video describes how to thaw and expand adult epithelial stem cells in the MimEX^™ System. It can be performed starting with either a MimEX^™ Stem Cell Line or from cell stocks cryopreserved from epithelial organ biopsy tissue.

In this protocol you will learn:

• Optimizing irradiated fibroblasts before plating stem cells
• General protocol for thawing and plating adult stem cells
• How to perform the MimEX^™ Cell Plating Procedure

Related Resources:
Learn More about MimEX^™ Tissue Model Systems
View MimEX^™ Cell Plating Protocol Video
View all protocol videos for MimEX^™ Tissue Models