MimEX™ Tissue Models System provides a platform for the isolation, expansion, and differentiation of adult epithelial stem cells into 3-D ex vivo organ tissue. This protocol video describes how to thaw and expand adult epithelial stem cells in the MimEX™ System. It can be performed starting with either a MimEX™ Stem Cell Line or from cell stocks cryopreserved from epithelial organ biopsy tissue.
In this protocol you will learn:
Optimizing irradiated fibroblasts before plating stem cells
General protocol for thawing and plating adult stem cells