Tips for Adult Stem Cell Differentiation using MimEX™ Reagents

MimEX™ Tissue Model Systems provide a robust system to generate accessible ex vivo 3-D tissue from epithelial-derived organs. This short video focuses on tips to optimize the differentiation of MimEX^™ Tissue in an air-liquid interface. This includes helpful information on harvesting adult stem cells for differentiation, optimizing the setup of transwell inserts, and monitoring and identifying successful versus unsuccessful differentiation in the air-liquid interface system.

In this protocol you will learn:

• Optimal harvesting density of adult stem cells
• Ideal structure of a healthy adult stem cell colony
• Troubleshooting fibroblast contamination when isolating adult stem cells from biopsy tissue
• Tips for working with transwell inserts
• Expected cytoarchitectural changes during adult stem cell differentiation
• Tips to identify successfully differentiating tissue

Related Resources:
Learn More about MimEX^™ Tissue Model Systems
View MimEX^™ Cell Plating Protocol Video
View all protocol videos for MimEX^™ Tissue Models