Blog

Cultrex® BME Coating for Adult Stem Cell Expansion and Differentiation

星期日, 一月 28, 2018 - 09:26

This protocol video demonstrates how to surface-coat cell culture plates and transwell inserts with Cultrex® Basement Membrane Extract, also called Cultrex® BME. Cultrex® BME is an extracellular matrix scaffold commonly used in stem cell culture and 3-dimensional (3-D) cell culture protocols, such as organoids and MimEX™ Tissue Model Systems. Using Cultrex® BME, MimEX Tissue Models System provides a platform for the isolation, expansion, and differentiation of adult epithelial stem cells into 3-D ex vivo organ tissue.

In this protocol you will learn:

  • Surface-coating protocol for Cultrex® BME
  • Optimal dilutions of Cultrex® BME for expansion and differentiation
  • General tips for working with Cultrex® BME
  • ...

Advanced Immunolabeling Reagents and Tools Now Available from Bio-Techne

星期三, 一月 24, 2018 - 10:29
Immunolabeling Reagents available from Bio-Techne

Bio-Techne Partners with Vector Laboratories To Provide Better Kits and Reagents for Immunostaining

Bio-Techne has announced a new offering of reagents and kits from Vector Laboratories, a leader in labeling and detection solutions. Beginning in October 2017, Bio-Techne began to distribute Vector Laboratories products to complement the over 40,000 primary antibodies from Novus Biologicals® validated for immunocytochemistry and immunohistochemistry.

Vector...

Expanding Cryopreserved Epithelial-derived Adult Stem Cells

星期六, 一月 20, 2018 - 10:04

MimEX Tissue Models System provides a platform for the isolation, expansion, and differentiation of adult epithelial stem cells into 3-D ex vivo organ tissue. This protocol video describes how to thaw and expand adult epithelial stem cells in the MimEX System. It can be performed starting with either a MimEX Stem Cell Line or from cell stocks cryopreserved from epithelial organ biopsy tissue.

In this protocol you will learn:

  • Optimizing irradiated fibroblasts before plating stem cells
  • General protocol for thawing and plating adult stem cells
  • How to perform the MimEX Cell Plating Procedure

 

Related Resources:
Learn More about MimEX Tissue Model Systems
...

Bio-Techne Go Everywhere Travel Grant - December 2017 Recipient Selected!

星期五, 一月 12, 2018 - 09:15
Bio-Techne travel grants

December 2017 Go Everywhere with Bio-Techne Travel Grant Awarded to a PhD Research Associate from the University of Texas at El Paso!

Bio-Techne launched its Go Everywhere Travel Grant Program which awards $1000 USD (or equivalent) to one researcher each month to attend the meeting of their choice. This grant program allows the researcher to attend more targeted meetings in their field and provides funding to support both small and large scientific associations that promote discovery and collaboration.

The recipient of the December 2017 grant is Jitian Li, a PhD Research Associate in the Department of Biological Science at the University of Texas at El Paso!

...

High Profile Papers Identify GFRAL as a GDF-15 Receptor and Novel Target for Obesity and Anorexia/Cachexia

星期三, 一月 10, 2018 - 10:16
GFRAL as a GDF

Growth Differentiation Factor 15 (GDF-15) has long been recognized as a circulating biomarker for a range of human disorders, and elevated levels have been associated with suppression of appetite. The receptor for this affect was recently revealed in several publications to be GDNF receptor-alpha-like (GFRAL), a distant member of the GDNF R family of proteins (1-4). The full receptor complex is composed of both Ret tyrosine kinase and GFRAL dimers. This newly discovered role for GFRAL places it in a position to act as a drug target for appetite regulation and obesity. This is an exciting finding given the great impact obesity-related disorders have on...

Stem Cells in the News - January 2018

星期三, 一月 3, 2018 - 09:58
Stem Cells in the News

We have captured this month's most interesting, innovative, and maybe some of the strangest examples of stem cells in the news from around the world.

AAAS

A Japanese research group form Kumamoto University’s Institute of Molecular Embryology and Genetics (IMG) have developed a method to recreate higher order kidney structures involving...

Webinar Short: B7-related Butryophilins as Immune Modulators

星期二, 十二月 26, 2017 - 08:41

A short description of Butyrophilin proteins produced by R&D Systems. These B7-related proteins may be checkpoint targets.

Butyrophilin Products

Recorded December 22, 2017

Neuroscience Research Review: Neuronal EphB1 Activates a Neuroprotective Astrocyte Phenotype

星期五, 十二月 15, 2017 - 08:36
Neuroprotective Astrocytes Induced by Neuronal EphB1

In the central nervous system (CNS), glia are key for maintaining tissue homeostasis. They function to support numerous processes including neurogenesis and neuronal survival, neural transmission, and immune surveillance. They also play a role in the initiation and progression of neuroinflammation and neurodegenerative disease. Astrocytes, the most abundant glial cell in the CNS, become activated in response to many insults, such as trauma, stroke, or neurodegenerative disease.1 Astrogliosis appears to be a complicated process as research is showing that the phenotype of reactive astrocytes may be defined by...

20 Years and Getting Stronger – The Ins and Outs of SUMOylation

星期二, 十二月 12, 2017 - 16:35

Posttranslational modification with Small Ubiquitin-related Modifier (SUMO) proteins is now established as one of the key regulatory protein modifications in eukaryotic cells. Hundreds of proteins involved in processes such as chromatin organization, transcription, DNA repair, macromolecular assembly and protein homeostasis, trafficking and signal transduction are subject to reversible sumoylation. It is hence not surprising that disease links are beginning to emerge and that interference with sumoylation is being considered for intervention. In this webinar, Dr. Frauke Melchior provides an overview of fundamental mechanisms and highlight recent developments in the analysis and physiology of sumoylation.

Click...

RNAScope® Technology Hits 1000 Publications

星期三, 十二月 6, 2017 - 07:49
Bio-Techne’s RNAScope

Bio-Techne’s RNAScope® Technology Hits 1000 Publications!

Bio-Techne is celebrating an exciting milestone for our RNAScope® assays from the Advanced Cell Diagnostics brand: 1000 publications citing the use of RNAScope® in situ hybridization (ISH) technology! We have announced this milestone in a press release on November 28, 2017.

With 400 of these publications in 2017 alone, this surge includes a notable increase in the use of the RNAScope technology in the pharmaceutical and biotechnology industries. This increase highlights awareness of the robust, sensitive, and specific ISH assay by...

Intestinal Lamina Propria Cell Markers

星期一, 十二月 4, 2017 - 16:31
Intestinal Lamina Proprial Cell Markers

R&D Systems Cell Marker Pages: The Easiest Way to Plan Your Next Experiment

One of the challenges of moving into a new research field or expanding your current field of study is determining the best markers to identify the cells you want. R&D Systems has been providing researchers with reliable tools for defining cell populations for over 30 years. Now we’ve added new Cell Marker Tools for Mucosal Immunity, Atherosclerosis, and Asthma on our website!

Each...

Quarterly Protein Product and Data Update: Edition #3

星期一, 十二月 4, 2017 - 12:26
Protein Structure Banner
 

New Products

recombinant GFRAL
GFRAL: The Receptor that Mediates the Metabolic Function of GDF-15 is Identified
Several high-profile papers have recently identified GFRAL as a receptor mediating GDF-15 effects on obesity and insulin resistance. R&D Systems’ new Recombinant Human GFRAL binds GDF-15 with an ED50 in the sub ng/mL range....

Stem Cells in the News - December 2017

星期一, 十二月 4, 2017 - 12:12
Stem Cells in the News

We have captured this month's most interesting, innovative, and maybe some of the strangest examples of stem cells in the news from around the world.

Science Daily

A team of researchers at Fred Hutchinson Cancer Research Center have identified a single, specific subset of adult stem cells that are...

RAS Oncoproteins: Therapeutic Vulnerabilities

星期五, 十二月 1, 2017 - 14:05

Adrienne Cox, Ph.D. Associate Professor, UNC Chapel Hill

The three human RAS↗ genes (HRAS↗, KRAS↗ and NRAS↗) encode four highly related RAS small GTPases↗ (HRAS, KRAS4A, KRAS4B and NRAS). RAS proteins function as GDP-GTP regulated binary on-off switches that regulate diverse cytoplasmic signaling networks. In cancer and developmental disorders (RASopathies), mutationally activated RAS proteins drive aberrant signal transduction. RAS proteins are also the founding...

How to Keep Calm and Monitor Cell Stress, Death, and Behavior

星期二, 十一月 21, 2017 - 14:57
How to Keep Calm and Monitor Cell Stress, Death, and Behavior

Measuring and monitoring cell health, stress, and behavior is a necessary part of using 2D and 3D cell culture models. This can be done qualitatively through microscopic assessment of cell structure, morphology, and confluency. Quantitative assessment of cell stress, death, and behavior is often needed when using 2D or 3D cell cultures for drug discovery, drug screening, and disease modeling. With the acquisition of Trevigen, Bio-Techne now has an extensive portfolio and unmatched expertise in assays and reagents for measuring cellular proliferation, viability, stress, DNA damage, and apoptosis. This blog showcases our expertise by highlighting the various resources and tools available through Bio-Techne.

...

Bio-Techne Go Everywhere Travel Grant - October Recipient Selected!

星期五, 十一月 17, 2017 - 14:33
Bio-Techne travel grants

October 2017 Go Everywhere with Bio-Techne Travel Grant Awarded to Post-Doc from University of Tampere!

Bio-Techne launched its Go Everywhere Travel Grant Program which awards $1000 USD (or equivalent) to 1 researcher each month to attend the meeting of their choice. This grant program allows the researcher to attend more targeted meetings in their field and provides funding to support both small and large scientific associations that promote discovery and collaboration.

The recipient of the October 2017 grant is Krista Rantanen, PhD, a Post-Doctoral Researcher in the Department of Automation Science and Engineering at the University of Tampere in Tampere, Finland!

...

Bio-Techne Awards Cancer Researcher $40,000 Grant

星期一, 十一月 13, 2017 - 21:58
Behren Get Hybridized Recipient from ACD Bio

Advanced Cell Diagnostics, a Bio-Techne Brand, completed a yearlong 'RNAscope ISH Get Hybridized' giveaway awarding Dr. Andreas Behren from the Olivia Newton-John Cancer Research (ONJCRI) Institute in Melbourne, Australia the grand prize of $40,000 grant to support his research in Cancer Immunobiology.

Several thousand researchers from around the world participated in the year-long giveaway by describing their research and its impact, as well as a potential role for RNAscope ISH.

The grant was presented at the Bio-Techne Wax Museum Party at the Society for Neuroscience 2017 Annual Meeting in Washington DC on November 13, 2017.

At the event, Dr....

5 Reasons Why the Cultrex Brand Is Essential for 3D Cell Culture

星期四, 十一月 9, 2017 - 14:56
Bio-Techne and Trevigen - Building Better 2D and 3D Culture Solutions

When Bio-Techne acquired Trevigen we expanded our offering of Cultrex® Basement Membrane Extract (BME) and Cultrex® Extracellular Matrix (ECM) Proteins, strengthening our portfolio of tools for 3D cell culture. Cultrex® BME and ECM are primed to become an integral set of scaffolding reagents for cancer and regenerative medicine research, specifically through the growth of organoids and...

CAR-TCR Summit 2017

星期三, 十一月 1, 2017 - 11:21
CAR TCR Summit 2017 - Seaport World Trade Center

By Janice Frias, Ph.D., Bio-Techne Scientist

The CAR-TCR Summit in Boston from September 5-8 could not have had a better venue than the Seaport World Trade Center. The space was the perfect size for a group like this. It was able to accommodate all the concurrent talks and expo, yet keep the attendees in still a rather intimate setting to encourage interaction. And a glance out the window at the Boston Harbor in between talks was a nice perk.

The summit was very timely in that the week prior, the Novartis CAR T therapy, Kymriah, received FDA approval to fight leukemia in children and young adults. This was a hot topic, sparking speculation about who is...

Stem Cells in the News - November 2017

星期一, 十月 30, 2017 - 15:16
Stem Cells in the News

We have captured this month's most interesting, innovative, and maybe some of the strangest examples of stem cells in the news from around the world.

USC News

With incidences of liver cancer steadily on the rise since 2003, it is important that we discover new routes of therapeutic options for patients. A team of...

Building Better 2D and 3D Culture Solutions - Bio-Techne and Trevigen

星期一, 十月 23, 2017 - 12:38
Bio-Techne and Trevigen - Building Better 2D and 3D Culture Solutions

On September 5, 2017 Bio-Techne proudly announced its acquisition of Trevigen, Inc. For over 25 years, Trevigen has developed innovative tools for generating and analyzing physiologic cell culture models, such as organoids, tumorspheres, and stem cell-derived cellular systems. These models are being used to make critical advancements in drug discovery, toxicology, regenerative medicine, cell therapy, and cancer research. With strong brands like the Cultrex® Basement Membrane Matrix and the CometAssay® for cell damage, the Trevigen product portfolio is an important addition to Bio-Techne....

Bio-Techne Go Everywhere Travel Grant - September Recipient Selected!

星期二, 十月 17, 2017 - 09:09
Bio-Techne travel grants

September 2017 Go Everywhere with Bio-Techne Travel Grant Awarded to Medical Biologist at Linköping University!

Bio-Techne launched its Go Everywhere Travel Grant Program which awards $1000 USD (or equivalent) to 1 researcher each month to attend the meeting of their choice. This grant program allows the researcher to attend more targeted meetings in their field and provides funding to support both small and large scientific associations that promote discovery and collaboration.

The recipient of the September 2017 grant is Helena Enocsson, a Medical Biologist in the Clinical and Experimental Medicine Department at Linköping University in Linköping, Sweden!

...

Immunology News: Researchers Report the Identification of a New Regulatory Innate Lymphoid Cell Subset that Suppresses Innate Intestinal Inflammation

星期五, 十月 13, 2017 - 07:37

Regulatory ILCs Suppress ILC1- and ILC3-Induced Innate Intestinal Inflammation

Regulatory ILCs Suppress ILC1- and ILC3-Induced Innate Intestinal Inflammation

Innate lymphoid cells (ILCs) are a recently described population of lymphocytes that play a critical role in innate immune responses and tissue remodeling. Multiple studies suggest that ILCs are particularly important for maintaining epithelial barriers and regulating immune responses at mucosal surfaces such as the lungs and intestines. Similar to T cells, ILCs consist of several subtypes that are characterized by the expression of different transcription factors and cytokines, and these correspond with the...

Luminex® Assay Ordering Tool: Take the Tour Now

星期二, 十月 3, 2017 - 11:50

Customer service numbers

1-800-343-7475 – USA, Canada, International

44 (0) 1235 529449 – EMEA

1-800-988-1270 – China

Find your local distributor

 

We’ve improved our Luminex® Assay Ordering Tool. It is now easier than ever to order the assays you want.

  • More Intuitive – We’ve streamlined the process starting from picking the species you want to easily...

Stem Cells in the News - October 2017

星期五, 九月 29, 2017 - 15:44
Stem Cells in the News

We have captured this month's most interesting, innovative, and maybe some of the strangest examples of stem cells in the news from around the world.

Journal of Experimental Medicine

While Zika virus infection can be detrimental to the developing brain, it may have a therapeutic application. Researchers at the University of California - San Diego and the...

页面

Blog Categories


Archives