This protocol video demonstrates how to surface-coat cell culture plates and transwell inserts with Cultrex® Basement Membrane Extract, also called Cultrex® BME. Cultrex® BME is an extracellular matrix scaffold commonly used in stem cell culture and 3-dimensional (3-D) cell culture protocols, such as organoids and MimEX™ Tissue Model Systems. Using Cultrex® BME, MimEX™ Tissue Models System provides a platform for the isolation, expansion, and differentiation of adult epithelial stem cells into 3-D ex vivo organ tissue.
In this protocol you will learn:
- Surface-coating protocol for Cultrex® BME
- Optimal dilutions of Cultrex® BME for expansion and differentiation
- General tips for working with Cultrex® BME ...