HA Tag Horseradish Peroxidase-conjugated Antibody

Recombinant Monoclonal Antibody
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HA Tag Horseradish Peroxidase-conjugated Antibody in Data
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Product Details

HA Tag Horseradish Peroxidase-conjugated Antibody Summary

Detects N-terminal and C-terminal HA-tagged proteins in Western blot.
Recombinant Monoclonal Rabbit IgG Clone # 1049F
Protein A or G purified from hybridoma culture supernatant
HA Tag Peptide
Accession # ABB51961
Supplied as a solution in PBS containing a preservative.
HRP(Excitation= nm, Emission= nm)


Recommended Concentration
Western Blot
1:1000-1:3000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of HA-tagged Proteins by Western Blot. View Larger

Detection of HA-tagged Proteins by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA and C-terminal HA-tagged LRRN5. PVDF membrane was probed with 1:1000-1:3000 dilution of Rabbit Anti-HA Tag Peptide HRP-conjugated Monoclonal Antibody (Catalog # HAM0601). Specific bands were detected for HA-tagged proteins at approximately 70 kDa and 77 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: HA Tag

Entrez Gene IDs
ABB51961.1 (Viral)
Alternate Names
HA Epitope Tag; HA Tag; HA; Hemagglutinin

Product Datasheets


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