Human HLA-DR Fluorescein-conjugated Antibody

FAB4869F 2 Citations Datasheet / COA / SDS

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FAB4869F-100
FAB4869F-025

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Human HLA-DR Fluorescein-conjugated Antibody in Data

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Citations (2)

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Human HLA-DR Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human HLA‑DR.

Source

Monoclonal Mouse IgG₁ Clone # L203

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

RPMI 8866 human lymphoblastoid cells
Accession # P01903

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of HLA‑DR in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human HLA-DR Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4869F) or (B) isotype control antibody (Catalog # IC002F) and Mouse anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: HLA-DR

HLA-DR is a transmembrane human major histocompatibility complex 2 (MHC II) family member and consists of a 34 kDa (alpha) subunit and one of several 28 kDa (beta) subunits. HLA-DR is expressed primarily by B cells and dendritic cells (DC), in which it binds peptides derived from internalized and processed antigenic proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD4⁺ T cells. This interaction is central to antigen specificity in the adaptive immune response. HLA-DR alleles, polymorphisms, and aberrant expression are linked to a variety of diseases including autoimmunity and cancer.

Long Name

Major Histocompatibility Complex Class II DR

Entrez Gene IDs

3122 (Human)

Alternate Names

FLJ51114; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen, DR alpha chain; HLADR; HLA-DR; HLA-DRA; HLA-DRA1; major histocompatibility complex, class II, DR alpha; MHC cell surface glycoprotein; MHC class II antigen DRA; MHC Class II DR; MLRW

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human HLA-DR Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Functional and molecular characterization of ex vivo cultured epiretinal membrane cells from human proliferative diabetic retinopathy.
Authors: Vereb Z, Lumi X, Andjelic S, Globocnik-Petrovic M, Urbancic M, Hawlina M, Facsko A, Petrovski G
Biomed Res Int, 2013;2013(0):492376.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Cultivation and characterization of cornea limbal epithelial stem cells on lens capsule in animal material-free medium.
Authors: Albert R, Vereb Z, Csomos K, Moe M, Johnsen E, Olstad O, Nicolaissen B, Rajnavolgyi E, Fesus L, Berta A, Petrovski G
PLoS ONE, 2012;7(10):e47187.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry