Human PD-L1 Quantikine ELISA Kit

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DB7H10
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PD-L1/B7-H1 ELISA Kit ELISA
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Human PD-L1 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Cell Lysates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Urine (100 uL)
Sensitivity
4.52 pg/mL
Assay Range
25.0 - 1,600 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant human B7-H1/PD-L1. This assay also recognizes natural cynomolgus monkey B7-H1/PD-L1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine® Human/Cynomolgus Monkey B7-H1/PD-L1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human/cynomolgus monkey B7-H1 in cell culture supernates, cell lysates, serum, plasma, and urine. It contains HEK293-expressed recombinant human B7-H1/PD-L1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human/cynomolgus monkey B7-H1/PD-L1 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human/cynomolgus monkey B7-H1/PD-L1.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 257 490 934 249 469 898
Standard Deviation 3.75 7.01 12.7 11.1 21.7 37.7
CV% 1.5 1.4 1.4 4.5 4.6 4.2

Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 260 458 884 244 466 878
Standard Deviation 4.25 6.83 14.7 15.9 25.8 66.4
CV% 1.6 1.5 1.7 6.5 5.5 7.6

Recovery

The recovery of human/cynomolgus monkey B7-H1/PD-L1 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 97-112
DYC002 (1X) (n=1) 96 90-100
EDTA Plasma (n=4) 90 79-100
Heparin Plasma (n=4) 85 76-95
Serum (n=4) 89 81-100
Urine (n=4) 109 100-116

Data Examples

Human PD-L1/B7-H1 ELISA Cell Culture Supernate/Cell Lysate/Serum/Plasma Standard Curve

Human PD-L1/B7-H1 ELISA Urine Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PD-L1/B7-H1

PD-L1, also known as B7-H1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells, keratinocytes, enothelial and intestinal epithelial cells, as well as a variety of carcinomas and melanoma. PD-L1 binds to T cell B7-1/CD80 and PD-1. It suppresses T cell activation and proliferation and induces the apoptosis of activated T cells. It plays a role in the development of immune tolerance by promoting T cell anergy and enhancing regulatory T cell development. PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells and inhibits the development of Th17 cells. In cancer, PD-L1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells.

Long Name:
Programmed Death Ligand 1
Entrez Gene IDs:
29126 (Human); 60533 (Mouse); 499342 (Rat); 484186 (Canine); 102145573 (Cynomolgus Monkey)
Alternate Names:
B7-H; B7H1; B7-H1; B7H1PDCD1L1; CD274 antigenMGC142294; CD274 molecule; CD274; PDCD1L1; PDCD1LG1; PDCD1LG1MGC142296; PDL1; PD-L1; PD-L1B7 homolog 1; PDL1PDCD1 ligand 1; programmed cell death 1 ligand 1; Programmed death ligand 1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citation for Human PD-L1 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Elevated serum soluble programmed cell death ligand 1 concentration as a potential marker for poor prognosis in small cell lung cancer patients with chemotherapy
    Authors: J Jin, J Si, Y Liu, H Wang, R Ni, J Wang
    Respir. Res., 2018;19(1):197.
    Species: Human
    Sample Types: Serum

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Reviews for Human PD-L1 Quantikine ELISA Kit

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Human PD-L1 Quantikine ELISA Kit
By Anonymous on 11/30/2018
Sample Tested: blood plasma

Human PD-L1 Quantikine ELISA Kit
By Anonymous on 10/10/2018
Sample Tested: Heparin Plasma

Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
By Anonymous on 04/04/2018
Sample Tested: Serum

Human/Cynomolgus Monkey PD-L1/B7-H1 Quantikine ELISA Kit
By Anonymous on 08/02/2017
Sample Tested: A375 human melanoma cell line