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Human Semaphorin 4D/CD100 PE-conjugated Antibody

Catalog #: FAB74701P
2 Citations Datasheet / COA / SDS
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FAB74701P

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Detection of Semaphorin 4D/CD100 in Jurkat Human Cell Line by Flow Cytometry.
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Human Semaphorin 4D/CD100 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Semaphorin 4D/CD100 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 4B, 4C, 4G, or recombinant mouse Semaphorin 4D is observed. In Western blots, no cross-reactivity with rhSemaphorin 4A, 6A, or 6C is observed.
Source
Monoclonal Mouse IgG1 Clone # 758726
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Semaphorin 4D/CD100
Met22-Arg734
Accession # Q92854
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Semaphorin 4D/CD100 antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Semaphorin 4D/CD100 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Semaphorin 4D/CD100 PE-conjugated Monoclonal Antibody (Catalog # FAB74701P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Semaphorin 4D/CD100

Semaphorin 4D (Sema4D)/CD100, previously Sem J, G or C-like 2, is a widely expressed 150 kDa type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861 amino acid (aa) Semaphorin 4D contains a 710 aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Semaphorin 4D shares 87% and 93% aa identity with human and rat Semaphorin 4D, respectively. A soluble, active 120 kDa form is produced proteolytically upon activation of B and T cells. Semaphorin 4D binds CD72 on B and dendritic cells and Plexin B1 elsewhere. In developing neurons, Semaphorin 4D assists in guidance. Semaphorin 4D is proangiogenic when expressed by tumor-associated macrophages.

Entrez Gene IDs
10507 (Human); 20354 (Mouse); 306790 (Rat)
Alternate Names
A8; BB18; CD100 antigen; CD100; CD100M-sema-G; COLL-4; FLJ33485; FLJ34282; FLJ39737; FLJ46484; immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D; MGC169138; MGC169141; M-sema-G; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D; SEMA4D; SEMAJ; Semaphorin 4D; semaphorin-4D

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Citations for Human Semaphorin 4D/CD100 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CD100 modulates cytotoxicity of CD8+ T cells in patients with acute myocardial infarction
    Authors: Y Li, L Qin, Q Bai, J Zhang, R Chen, K Song
    BMC immunology, 2021-02-16;22(1):13.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Interferon-?-induced CD100 on na�ve CD8(+) T cells enhances antiviral responses to hepatitis C infection through CD72 signal transduction
    Authors: BJ Li, Y He, Y Zhang, YH Guo, Y Zhou, PX Zhang, W Wang, JR Zhao, JG Li, WZ Zuo, C Fan, ZS Jia
    J. Int. Med. Res, 2017-01-12;45(1):89-100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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