INSTA-Blot Multi-species Liver, Lung, Spleen Tissues

Novus Biologicals | Catalog # NBP2-30117

Novus Biologicals
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Product Summary for INSTA-Blot Multi-species Liver, Lung, Spleen Tissues

Ready-to-use PVDF membrane with 11 human, mouse and rat tissue lysates (20 ug total protein per lane).
INSTA-Blot™ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Multi-species Liver, Lung, Spleen Tissues NBP2-30117 is a simple and fast solution for screening proteins from various human, mouse and rat tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot™, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.

Product Specifications

Application Notes

Human tissues are from donors with no known disease. Mouse tissues are from Balb/c mice. Rat tissues are from Sprague Dawley rats. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).

Scientific Data Images for INSTA-Blot Multi-species Liver, Lung, Spleen Tissues

SDS-PAGE: INSTA-Blot Multi-species Liver, Lung, Spleen Tissues [NBP2-30117]

SDS-PAGE: INSTA-Blot Multi-species Liver, Lung, Spleen Tissues [NBP2-30117]

SDS-Page: INSTA-Blot Multi-species Liver, Lung, Spleen Tissues [NBP2-30117] - Approximately 20 ug per lane of tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2. human liver; 3. mouse liver; 4. rat liver; 5. Molecular weight marker; 6. human lung; 7. mouse lung; 8. rat lung; 9. Molecular weight marker; 10. human spleen; 11. mouse spleen; 12. rat spleen.

Formulation, Preparation, and Storage

Preparation Method

INSTA-Blot™ Multi-species Liver, Lung, Spleen Tissues NBP2-30117 contains denatured proteins from tissue lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% TrisGlycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot™ is provided ready-to-use in your western blot protocol.

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at room temperature.

Background: INSTA-Blot Multi-species Liver, Lung, Spleen Tissues

Alternate Names

INSTA Blot Multi-species Liver, Lung, Spleen Tissues, INSTA-Blot Multi-species Liver, Lung, Spleen, INSTABlot Multi-species Liver, Lung, Spleen Tissues

Additional INSTA-Blot Multi-species Liver, Lung, Spleen Tissues Products

Product Documents for INSTA-Blot Multi-species Liver, Lung, Spleen Tissues

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for INSTA-Blot Multi-species Liver, Lung, Spleen Tissues

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for INSTA-Blot Multi-species Liver, Lung, Spleen Tissues (NBP2-30117):

INSTA-Blot Multi-species Liver, Lung, Spleen Tissues:
Material Safety Data Sheet for Non Hazardous Products

Hazard Information
Chemical Name: Non hazardous products.
Chemical Formula: N/A
CAS Number: N/A
EEC-No: N/A

Hazard Identification
None

First Aid Measures
Eye Contact: None
Skin Contact: None
Inhalation: None
Ingestion: None

Accidental Release Measures
This product either does not contain hazardous constituents or the concentration of all chemical constituents are below the regulatory threshold limits described by Occupational Safety Health Administration Hazard Communication Standard 29 CFR 1910.1200 and the European Directive 91/155/EEC. 88/379/EEC, and 67/546/EEC.

Handling and Storage
Exposure Controls / Personal Protection
Other Precautions: None

Physical and Chemical Properties
Form: N/A
Color: N/A
Odor: N/A
Melting Point: N/A
Boiling Temperature: N/A
Density: N/A
Vapor Pressure: N/A
Solubility in Water: N/A
Flash Point: N/A
Explosion limits: N/A
Ignition Temperature: N/A

FAQs

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