Mouse CD8 alpha Antibody

R&D Systems | Catalog # MAB11723

R&D Systems
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG Clone # 1104518
View all CD8 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line, NS0-derived mouse CD8
Lys28-Tyr196
Accession # P01731

Specificity

Detects recombinant mouse CD8alpha protein in Direct ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG

Scientific Data Images for Mouse CD8 alpha Antibody

Detection of Mouse CD8 alpha by Western Blot.

Western Blot shows lysates of mouse thymus tissue. PVDF membrane was probed with 2 µg/ml of Rat Anti-Mouse CD8 alpha Monoclonal Antibody (Catalog # MAB11723) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for CD8 alpha at approximately 30 and 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Mouse CD8 alpha Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Mouse thymus tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: CD8

CD8, also known as Ly-2, is a heterodimeric glycoprotein consisting of an alpha and beta chain. It is expressed on cytolytic T cells and functions in conjunction with the T cell receptor in the recognition of MHC/peptide complexes. Mouse CD8 (containing an alpha /Ly-2 or alpha ′/Lyt-2 chain) is an antigen co‑receptor on the T cell surface which interacts with MHC I molecules on antigen presenting cells (1). CD8 alpha beta heterodimer is expressed on a subpopulation of mature T cells (2, 3). CD8 alpha, without CD8 beta, has been detected on subsets of gamma delta TCR-bearing T cells (4), intestinal intrathymic lymphocytes (5, 6) and dendritic cells (7, 8).

References

  1. Bierer, B.E. et al. (1989) Annu. Rev. Immunol. 7:579.
  2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.
  3. Hayakawa, K. et al. (1994) Science 263:1131.
  4. MacDonald, H.R. et al. (1990) Eur. J. Immunol. 20:927.
  5. Rocha, B. et al. (1992) Immunol. Today 13:449.
  6. Wang, J. and J.R. Klein (1994) Science 265:1860.
  7. Vermec, D. et al. (1992) J. Exp. Med. 176:47.
  8. Suss, G. and K. Shortman (1996) J. Exp. Med. 183:1789.

Alternate Names

CD8, CD8A

Entrez Gene IDs

925 (Human); 12525 (Mouse); 24930 (Rat); 102124337 (Cynomolgus Monkey); 493799 (Feline)

Gene Symbol

CD8A

UniProt

P01731

Additional CD8 Products

Product Documents for Mouse CD8 alpha Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD8 alpha Antibody

For research use only

Customer Reviews for Mouse CD8 alpha Antibody

There are currently no reviews for this product. Be the first to review Mouse CD8 alpha Antibody and earn rewards!

Have you used Mouse CD8 alpha Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies