Mouse Magnetic Luminex Assay
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Build NowMouse Magnetic Luminex Assay Summary
Assay Type | Magnetic bead-based multiplex assay for the Luminex® platform |
Format | 1 x 96-well microplate and Magnetic antibody-coated beads |
Species | Mouse |
Analytes Detected | Please see analyte list in assay customization tool below. |
Performance Validation | Luminex Assays are validated for use with cell culture supernatants, tissue lysates, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity. |
Make your selections below to configure your assay
Assays
Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.
If you run into bead region incompatibilities, send your Luminex code to our team and find out more.
How would you like these analytes mixed?
Would you like to split based on dilution?
Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.
Magnetic Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.
- Simultaneously profile up to 50 analytes of your choice in one sample
- Choose from a wide menu of analytes, including many unique analytes
- Requires a small sample volume (<50 µL)
- Run your assay in just 3-4 hours
- Mass-calibrated standards for consistent results with every new lot of material
- Enough reagents for one 96-well plate
- Quick turn-around time: Kits are typically shipped within 5-10 days of ordering.*
Please allow additional time for international orders.
- Premixed cocktail of antibody-coated Magnetic beads
- Premixed cocktail of biotinylated detection antibody
- Standard Cocktail(s)
- Bead Diluent
- Biotin Antibody Diluent
- Standard/Sample Diluent
- Wash Buffer
- Streptavidin-PE
- One flat-bottom 96-well Microplate
- Foil Plate Sealers (4)
- Mixing Bottle
- Certificate of Analysis**
**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.
Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.
Data Example
Product Datasheets
Citations for Mouse Magnetic Luminex Assay
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Genetic Deficiency of the Histamine H4-Receptor Reduces Experimental Colorectal Carcinogenesis in Mice
Authors: B Schirmer, T Rother, I Bruesch, A Bleich, C Werlein, D Jonigk, R Seifert, D Neumann
Cancers (Basel), 2020;12(4):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Kinin B1 receptor: a potential therapeutic target in sepsis-induced vascular hyperpermeability
Authors: S Ruiz, F Vardon-Bou, M Buléon, C Guilbeau-F, MH Séguelas, JM Conil, JP Girolami, I Tack, V Minville
J Transl Med, 2020;18(1):174.
Species: Mouse
Sample Types: Serum
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Smooth-muscle-derived WNT5A augments allergen-induced airway remodelling and Th2 type inflammation
Authors: T Koopmans, L Hesse, MC Nawijn, K Kumawat, MH Menzen, I Sophie T B, R Smits, ERM Bakker, M van den Be, GH Koppelman, V Guryev, R Gosens
Sci Rep, 2020;10(1):6754.
Species: Mouse
Sample Types: Tissue Homogenates
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A combination of genetics and microbiota influences the severity of the obesity phenotype in diet-induced obesity
Authors: M Smoczek, M Vital, D Wedekind, M Basic, NH Zschemisch, DH Pieper, A Siebert, A Bleich, M Buettner
Sci Rep, 2020;10(1):6118.
Species: Mouse
Sample Types: Serum
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ADAR1 Alleviates Inflammation in a Murine Sepsis Model via the ADAR1-miR-30a-SOCS3 Axis
Authors: Z Shangxun, L Junjie, Z Wei, W Yutong, J Wenyuan, L Shanshou, W Yanjun, W Qianmei, F Zhusheng, Y Chaoping, Z Ran, Y Wen, H Yang
Mediators Inflamm., 2020;2020(0):9607535.
Species: Mouse
Sample Types: Serum
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Anti-HER2 induced myeloid cell alterations correspond with increasing vascular maturation in a murine model of HER2+ breast cancer
Authors: MJ Bloom, AM Jarrett, TA Triplett, AK Syed, T Davis, TE Yankeelov, AG Sorace
BMC Cancer, 2020;20(1):359.
Species: Mouse
Sample Types: Cell Lysates
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Commiphora Extract Mixture Ameliorates Monosodium Iodoacetate-Induced Osteoarthritis
Authors: D Lee, MK Ju, H Kim
Nutrients, 2020;12(5):.
Species: Mouse
Sample Types: Serum
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Maintaining immunogenicity of blood stage and sexual stage subunit malaria vaccines when formulated in combination
Authors: EM Parzych, K Miura, CA Long, JM Burns
PLoS ONE, 2020;15(4):e0232355.
Species: Mouse
Sample Types: Cell Culture Supernates
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HCT116 colorectal liver metastases exacerbate muscle wasting in a mouse model for the study of colorectal cancer cachexia
Authors: JR Huot, LJ Novinger, F Pin, A Bonetto
Dis Model Mech, 2020;0(0):.
Species: Mouse
Sample Types: Plasma
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Papaver nudicaule (Iceland poppy) alleviates lipopolysaccharide-induced inflammation through inactivating NF-?B and STAT3
Authors: JH Oh, M Yun, D Park, IJ Ha, CK Kim, DW Kim, EO Kim, SG Lee
BMC Complement Altern Med, 2019;19(1):90.
Species: Mouse
Sample Types: Cell Culture Supernates
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Performance of a glucose-reactive enzyme-based biofuel cell system for biomedical applications
Authors: WY Jeon, JH Lee, K Dashnyam, YB Choi, TH Kim, HH Lee, HW Kim, HH Kim
Sci Rep, 2019;9(1):10872.
Species: Mouse
Sample Types: Whole Cells
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AMPK as a potential pharmacological target for alleviating LPS-induced acute lung injury partly via NLRC4 inflammasome pathway inhibition
Authors: Y He, K Xu, Y Wang, X Chao, B Xu, J Wu, J Shen, W Ren, Y Hu
Exp. Gerontol., 2019;125(0):110661.
Species: Mouse
Sample Types: BALF
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Ovalbumin-sensitized mice have altered airway inflammation to agriculture organic dust
Authors: KJ Warren, JD Dickinson, AJ Nelson, TA Wyatt, DJ Romberger, JA Poole
Respir. Res., 2019;20(1):51.
Species: Mouse
Sample Types: BALF
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Adipose Tissue Regulates Pulmonary Pathology during TB Infection
Authors: JP Ayyappan, U Ganapathi, K Lizardo, C Vinnard, S Subbian, D Perlin, JF Nagajyothi
MBio, 2019;10(2):.
Species: Mouse
Sample Types: Serum
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Apicidin Attenuates MRSA Virulence through Quorum-Sensing Inhibition and Enhanced Host Defense
Authors: CP Parlet, JS Kavanaugh, HA Crosby, HA Raja, T El-Elimat, DA Todd, CJ Pearce, NB Cech, NH Oberlies, AR Horswill
Cell Rep, 2019;27(1):187-198.e6.
Species: Mouse
Sample Types: Tissue Homogenates
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Inhibition of lymphatic proliferation by the selective VEGFR-3 inhibitor SAR131675 ameliorates diabetic nephropathy in db/db mice
Authors: SD Hwang, JH Song, Y Kim, JH Lim, MY Kim, EN Kim, YA Hong, S Chung, BS Choi, YS Kim, CW Park
Cell Death Dis, 2019;10(3):219.
Species: Mouse
Sample Types: Serum
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Th1-biased immunoadjuvant effect of the recombinant B subunit of an Escherichia coli heat-labile enterotoxin on an inactivated PRRSV antigen via intranasal immunization in mice
Authors: F Su, L Xu, Y Xue, J Li, Y Fu, B Yu, S Wang, X Yuan
J. Vet. Med. Sci., 2019;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
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EphA2 Is a Neutrophil Receptor for Candida albicans that Stimulates Antifungal Activity during Oropharyngeal Infection
Authors: M Swidergall, NV Solis, Z Wang, QT Phan, ME Marshall, MS Lionakis, E Pearlman, SG Filler
Cell Rep, 2019;28(2):423-433.e5.
Species: Mouse
Sample Types: Tissue Homogenates
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Transcriptomic profiling of the myeloma bone-lining niche reveals BMP signalling inhibition to improve bone disease
Authors: S Gooding, SWZ Olechnowic, EV Morris, AE Armitage, J Arezes, J Frost, E Repapi, JR Edwards, N Ashley, C Waugh, N Gray, E Martinez-H, PJ Lim, SR Pasricha, H Knowles, AJ Mead, K Ramasamy, H Drakesmith, CM Edwards
Nat Commun, 2019;10(1):4533.
Species: Mouse
Sample Types: Bone Marrow Plasma
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Comprehensive Analysis of the Safety Profile of a Single-Stranded RNA Nano-Structure Adjuvant
Authors: HJ Park, HL Ko, DH Won, DB Hwang, YS Shin, HW Kwak, HJ Kim, JW Yun, JH Nam
Pharmaceutics, 2019;11(9):.
Species: Mouse
Sample Types: Serum
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BET Proteins Are Required for Transcriptional Activation of the Senescent Islet Cell Secretome in Type 1 Diabetes
Authors: PJ Thompson, A Shah, H Apostolopo, A Bhushan
Int J Mol Sci, 2019;20(19):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Depletion of Bone Marrow-Derived Fibrocytes Attenuates TAA-Induced Liver Fibrosis in Mice
Authors: F Hempel, M Roderfeld, R Savai, A Sydykov, K Irungbam, R Schermuly, R Voswinckel, K Köhler, Y Churin, L Kiss, J Bier, J Pons-Kühne, E Roeb
Cells, 2019;8(10):.
Species: Mouse
Sample Types: Tissue Homogenates
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Stereotactic Body Radiation and Interleukin-12 Combination Therapy Eradicates Pancreatic Tumors by Repolarizing the Immune Microenvironment
Authors: BN Mills, KA Connolly, J Ye, JD Murphy, TP Uccello, BJ Han, T Zhao, MG Drage, A Murthy, H Qiu, A Patel, NM Figueroa, CJ Johnston, PA Prieto, NK Egilmez, BA Belt, EM Lord, DC Linehan, SA Gerber
Cell Rep, 2019;29(2):406-421.e5.
Species: Mouse
Sample Types: Tissue Homogenates
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Loading ceftriaxone, vancomycin, and Bifidobacteria bifidum TMC3115 to neonatal mice could differently and consequently affect intestinal microbiota and immunity in adulthood
Authors: R Cheng, J Guo, F Pu, C Wan, L Shi, H Li, Y Yang, C Huang, M Li, F He
Sci Rep, 2019;9(1):3254.
Species: Mouse
Sample Types: Serum
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Allergic inflammation alters the lung microbiome and hinders synergistic co-infection with H1N1 influenza virus and Streptococcus pneumoniae in C57BL/6 mice
Authors: KS LeMessurie, AR Iverson, TC Chang, M Palipane, P Vogel, JW Rosch, AE Samarasing
Sci Rep, 2019;9(1):19360.
Species: Mouse
Sample Types: BALF
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FOXO3a regulates rhinovirus-induced innate immune responses in airway epithelial cells
Authors: J Gimenes-Ju, N Owuar, HR Vari, W Li, N Xander, S Kotnala, US Sajjan
Sci Rep, 2019;9(1):18180.
Species: Mouse
Sample Types: BALF
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Distinct alterations of gut morphology and microbiota characterize accelerated diabetes onset in non-obese diabetic mice
Authors: MC Simon, AL Reinbeck, C Wessel, J Heindirk, T Jelenik, K Kaul, J Arreguin-C, A Strom, M Blaut, F Bäckhed, V Burkart, M Roden
J. Biol. Chem., 2019;0(0):.
Species: Mouse
Sample Types: Serum
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Blocking connexin43 hemichannels protects mice against tumour necrosis factor-induced inflammatory shock
Authors: T Delvaeye, MAJ De Smet, S Verwaerde, E Decrock, A Czekaj, RE Vandenbrou, K Lemeire, A Gonçalves, W Declercq, P Vandenabee, DV Krysko, L Leybaert
Sci Rep, 2019;9(1):16623.
Species: Mouse
Sample Types: Plasma
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Neutrophil Recruitment to Noninvasive MRSA at the Stratum Corneum of Human Skin Mediates Transient Colonization
Authors: A Schulz, L Jiang, L de Vor, M Ehrström, F Wermeling, L Eidsmo, K Melican
Cell Rep, 2019;29(5):1074-1081.e5.
Species: Mouse
Sample Types: Tissue Homogenates
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Physiology and effects of nucleosides in mice lacking all four adenosine receptors
Authors: C Xiao, N Liu, KA Jacobson, O Gavrilova, ML Reitman
PLoS Biol., 2019;17(3):e3000161.
Species: Mouse
Sample Types: Plasma
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CXCL17-derived CD11b+Gr-1+ myeloid-derived suppressor cells contribute to lung metastasis of breast cancer through platelet-derived growth factor-BB
Authors: YL Hsu, MC Yen, WA Chang, PH Tsai, YC Pan, SH Liao, PL Kuo
Breast Cancer Res., 2019;21(1):23.
Species: Mouse
Sample Types: Cell Culture Supernates
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Effectiveness of two different dose administration regimens of an IL-15 superagonist complex (ALT-803) in an orthotopic bladder cancer mouse model
Authors: H Furuya, OTM Chan, I Pagano, C Zhu, N Kim, R Peres, K Hokutan, S Alter, P Rhode, CJ Rosser
J Transl Med, 2019;17(1):29.
Species: Mouse
Sample Types: Urine
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4-1BB enhancement of CAR T function requires NF-?B and TRAFs
Authors: G Li, JC Boucher, H Kotani, K Park, Y Zhang, B Shrestha, X Wang, L Guan, N Beatty, D Abate-Daga, ML Davila
JCI Insight, 2018;3(18):.
Species: Mouse
Sample Types: Cell Culture Supernates
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Extracellular signal regulated kinase 5 and inflammasome in progression of mesothelioma
Authors: JK Thompson, A Shukla, AL Leggett, PB Munson, JM Miller, MB MacPherson, SL Beuschel, HI Pass, A Shukla
Oncotarget, 2018;9(1):293-305.
Species: Mouse
Sample Types: Peritoneal Lavage Fluid
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Serum amyloid A3 is required for normal weight and immunometabolic function in mice
Authors: JL Ather, ME Poynter
PLoS ONE, 2018;13(2):e0192352.
Species: Mouse
Sample Types: Cell Culture Supernates
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Prophylactic efficacy of orally administered Bacillus poly-?-glutamic acid, a non-LPS TLR4 ligand, against norovirus infection in mice
Authors: W Lee, M Kim, SH Lee, HG Jung, JW Oh
Sci Rep, 2018;8(1):8667.
Species: Mouse
Sample Types: Serum
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Chronic features of allergic asthma are enhanced in the absence of resistin-like molecule-beta
Authors: KS LeMessurie, M Palipane, M Tiwary, B Gavin, AE Samarasing
Sci Rep, 2018;8(1):7061.
Species: Mouse
Sample Types: Tissue Homogenates
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Leptin Signaling Mediates Obesity-associated CSC Enrichment and EMT in Preclinical TNBC Models
Authors: LW Bowers, EL Rossi, SB McDonell, SS Doerstling, SA Khatib, CG Lineberger, JE Albright, X Tang, LA deGraffenr, SD Hursting
Mol. Cancer Res., 2018;0(0):.
Species: Mouse
Sample Types: Serum
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Caspase-8 deficiency in mouse embryos triggers chronic RIPK1-dependent activation of inflammatory genes, independently of RIPK3
Authors: TB Kang, JS Jeong, SH Yang, A Kovalenko, D Wallach
Cell Death Differ., 2018;0(0):.
Species: Mouse
Sample Types: Plasma
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Synergistic actions of FGF2 and bone marrow transplantation mitigate radiation-induced intestinal injury
Authors: BH Kim, HW Jung, SH Seo, H Shin, J Kwon, JM Suh
Cell Death Dis, 2018;9(3):383.
Species: Mouse
Sample Types: Serum
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RNA sequencing analysis reveals quiescent microglia isolation methods from postnatal mouse brains and limitations of BV2 cells
Authors: Y He, X Yao, N Taylor, Y Bai, T Lovenberg, A Bhattachar
J Neuroinflammation, 2018;15(1):153.
Species: Mouse
Sample Types: Cell Culture Supernates
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Lack of vitamin D signalling per se does not aggravate cardiac functional impairment induced by myocardial infarction in mice
Authors: K Ford, N Latic, S Slavic, U Zeitz, M Dolezal, O Andrukhov, RG Erben, O Andrukhova
PLoS ONE, 2018;13(10):e0204803.
Species: Mouse
Sample Types: Serum
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Systemic vascular leakage induced in mice by Russell's viper venom from Pakistan
Authors: A Rucavado, T Escalante, E Camacho, JM Gutiérrez, JW Fox
Sci Rep, 2018;8(1):16088.
Species: Mouse
Sample Types: Plasma
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A requirement for slc15a4 in imiquimod-induced systemic inflammation and psoriasiform inflammation in mice
Authors: AD Griffith, AK Zaidi, A Pietro, M Hadiono, JS Yang, R Davis, DL Popkin
Sci Rep, 2018;8(1):14451.
Species: Mouse
Sample Types: Plasma
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A novel, multiple-antigen pneumococcal vaccine protects against lethal Streptococcus pneumoniae challenge
Authors: WY Chan, C Entwisle, G Ercoli, E Ramos-Sevi, A McIlgorm, P Cecchini, C Bailey, O Lam, G Whiting, N Green, D Goldblatt, JX Wheeler, JS Brown
Infect. Immun., 2018;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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The effect of the cholinergic anti-inflammatory pathway on collagen-induced arthritis involves the modulation of dendritic cell differentiation
Authors: D Liu, T Li, H Luo, X Zuo, S Liu, S Wu
Arthritis Res. Ther., 2018;20(1):263.
Species: Mouse
Sample Types:
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The Interaction of miR-378i-Skp2 Regulates Cell Senescence in Diabetic Nephropathy
Authors: YC Tsai, PL Kuo, MC Kuo, WW Hung, LY Wu, WA Chang, PH Wu, SC Lee, HC Chen, YL Hsu
J Clin Med, 2018;7(12):.
Species: Mouse
Sample Types: Urine
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Lack of Histamine H4-Receptor Expression Aggravates TNBS-Induced Acute Colitis Symptoms in Mice
Authors: EJ Wunschel, B Schirmer, R Seifert, D Neumann
Front Pharmacol, 2017;8(0):642.
Species: Mouse
Sample Types: Tissue Homogenates
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Cannabinoid Receptor 2 Modulates Neutrophil Recruitment in a Murine Model of Endotoxemia
Authors: TS Kapellos, C Recio, DR Greaves, AJ Iqbal
Mediators Inflamm., 2017;2017(0):4315412.
Species: Mouse
Sample Types: Serum
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CDK4/6 Inhibition Augments Anti-Tumor Immunity by Enhancing T Cell Activation
Authors: J Deng, ES Wang, RW Jenkins, S Li, R Dries, K Yates, S Chhabra, W Huang, H Liu, AR Aref, E Ivanova, CP Paweletz, M Bowden, CW Zhou, GS Herter-Spr, JA Sorrentino, JE Bisi, PH Lizotte, AA Merlino, MM Quinn, LE Bufe, A Yang, Y Zhang, H Zhang, P Gao, T Chen, ME Cavanaugh, AJ Rode, E Haines, PJ Roberts, JC Strum, WG Richards, JH Lorch, S Parangi, V Gunda, GM Boland, R Bueno, S Palakurthi, GJ Freeman, J Ritz, WN Haining, NE Sharpless, H Arthanari, GI Shapiro, DA Barbie, NS Gray, KK Wong
Cancer Discov, 2017;0(0):.
Species: Mouse
Sample Types: X Lavage Fluid
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Cytokine treatment optimises the immunotherapeutic effects of umbilical cord-derived MSC for treatment of inflammatory liver disease
Authors: SFH de Witte, AM Merino, M Franquesa, T Strini, JAA van Zoggel, SS Korevaar, F Luk, M Gargesha, L O'Flynn, D Roy, SJ Elliman, PN Newsome, CC Baan, MJ Hoogduijn
Stem Cell Res Ther, 2017;8(1):140.
Species: Mouse
Sample Types: Serum
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The yeast form of the fungus Candida albicans promotes persistence in the gut of gnotobiotic mice
Authors: L Böhm, S Torsin, SH Tint, MT Eckstein, T Ludwig, JC Pérez
PLoS Pathog., 2017;13(10):e1006699.
Species: Mouse
Sample Types: Tissue Homogenates
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Lactobacillus paracasei feeding improves immune control of influenza infection in mice
Authors: N Belkacem, N Serafini, R Wheeler, M Derrien, L Boucinha, A Couesnon, N Cerf-Bensu, I Gomperts B, JP Di Santo, MK Taha, R Bourdet-Si
PLoS ONE, 2017;12(9):e0184976.
Species: Mouse
Sample Types: Tissue Homogenates
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Cell autonomous or systemic EGFR blockade alters the immune-environment in squamous cell carcinomas
Int J Cancer, 2016;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Toll-like receptor 2 activation depends on lipopeptide shedding by bacterial surfactants
Nat Commun, 2016;7(0):12304.
Species: Mouse
Sample Types: Serum
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Factor XI deficiency enhances the pulmonary allergic response to house dust mite in mice independent of factor XII
Authors: Ingrid Stroo
Am. J. Physiol. Lung Cell Mol. Physiol, 2016;0(0):ajplung.00320.
Species: Mouse
Sample Types: Tissue Homogenates
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Atypical chemokine receptor ACKR2 controls branching morphogenesis in the developing mammary gland
Authors: Gerard J Graham
Development, 2016;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
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Positive Prehabilitative Effect of Intense Treadmill Exercise for Ameliorating Cancer Cachexia Symptoms in a Mouse Model
J Cancer, 2016;7(15):2378-2387.
Species: Mouse
Sample Types: Plasma
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Nrf2 Modulates Host Defense during Streptococcus pneumoniae Pneumonia in Mice
Authors: JC Gomez, H Dang, JR Martin, CM Doerschuk
J. Immunol, 2016;197(7):2864-79.
Species: Mouse
Sample Types: BALF
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Complement Component 3 Regulates IFN-? Production by Plasmacytoid Dendritic Cells following TLR7 Activation by a Plant Virus-like Nanoparticle
Authors: Alain Lamarre
J. Immunol., 2016;0(0):.
Species: Mouse
Sample Types: Serum
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CF Lung Infection and Inflammation: IL-17 Pathophysiology and Therapeutic Intervention
Authors: Daniel Hsu
Infect Immun, 2016;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
FAQs
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Can this assay be used for analysis of tissue homogenates?
This assay has been validated for use with tissue lysates, and a protocol is noted in the product insert for preparation of the tissue lysate. The assay has not been validated for use with tissue homogenates. In order to validate tissue homogenate for this assay, a good starting point would be homogenizing in a non-denaturing buffer with protease inhibitors. We recommend validating this sample type with Spike/Recovery and Linearity testing. Please contact Technical Support at techsupport@bio-techne.com with additional questions.
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What is the reason for the recommendation to use 35 µL sample volume, instead of the default 50 µL volume, for Luminex multiplex containing > 25 analytes, when assayed on Luminex MAGPIX® analyzer?
R&D Systems recommend 35 µL sample volume for > 25 analytes to control the number of beads in the sample load when using Luminex MAGPIX® analyzer. Luminex MAGPIX® analyzer is a non-flow-based reader which utilizes a magnet to hold the magnetic microparticles in a monolayer in a chamber where the beads are imaged and then processed by the software to generate data. Since MAGPIX® system pushes the entire Sample Load Volume into the chamber, multiplex assay containing > 25 analytes may overload the chamber capacity with 50 µL sample load default setting. The captured images can have too many beads in them to give accurate results, and some of the analytes/regions may present lower than desired bead counts.
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