Mouse Tyro3/Dtk Biotinylated Antibody (Clone 109646)

Name: Mouse Tyro3/Dtk Biotinylated Antibody (Clone 109646)
Brand: R&D Systems
SKU: BAM759
Rating: 5 (1 reviews)

Catalog #: BAM759 1 Review Datasheet / COA / SDS

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BAM759

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Tyro3/Dtk Biotinylated Antibody (Clone 109646) Summary

Species Reactivity

Mouse

Specificity

Recognizes the extracellular domain of mouse Dtk. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Dtk, recombinant mouse (rm) Mer, or rmAxl is observed.

Source

Monoclonal Rat IgG₁ Clone # 109646

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Dtk
Met1-Ser418
Accession # P55144

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse Dtk Fc Chimera (Catalog # 759-DT)

Flow Cytometry

2.5 µg/10⁶ cells

Mouse embryoid bodies differentiated from D3 embryonic stem cells

Immunohistochemistry

8-25 µg/mL

Immersion fixed frozen sections of mouse embryo (E15)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tyro3/Dtk

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt) and Mer (human and mouse homologues of chicken c-Eyk) constitute a new receptor tyrosine kinase subfamily. The extracellular domain of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. All three receptors bind the vitamin K-dependent protein growth-arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. The binding affinities for Gas6 is in the order of Axl > Dtk > Mer. Gas6 binding induces tyrosine phosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis. Dtk is widely expressed during embryonic development. In adults, Dtk is predominantly expressed in neurons in restricted regions of the brain.

Long Name

Tyrosine Protein Kinase Receptor TYRO3

Entrez Gene IDs

7301 (Human); 22174 (Mouse)

Alternate Names

Brt; BYK; Dtk; EC 2.7.10; EC 2.7.10.1; FLJ16467; Rse; Sky; Tif; TYRO3 protein tyrosine kinase; Tyro3; Tyrosine-protein kinase byk; Tyrosine-protein kinase DTK; tyrosine-protein kinase receptor TYRO3; Tyrosine-protein kinase RSE; Tyrosine-protein kinase SKY