Accomplish more in your day without compromising quality. The Quantikine QuicKit ELISAs provide quick, accurate quantitation of proteins in serum, plasma, and cell culture supernates. Unlike traditional ELISAs, QuicKit ELISAs have a fast, simplified protocol that only takes 90 minutes to results with just one wash step.
Quantikine QuicKit ELISAs are extensively optimized to reduce matrix effects that are commonly seen with complex sample matrices. The following sample types have been validated for use in these ELISA kits:
These colorimetric ready-to-use ELISAs come with all components necessary to run your assay. Kit contents may vary slightly. Please refer to product-specific inserts for a complete list of contents for each individual assay:
These assays utilize an anti-tag coated microplate. Standards, samples, and controls are added to plate, followed by subsequent addition of an antibody cocktail. Following a 1 hour incubation, plates are washed and substrate is added and incubated for 20 minutes. Stop solution is then added and plates are read using a standard microplate reader.
Although Quantikine® QuicKitTM ELISAs produce results in a fraction of the time of traditional ELISAs, quality data is not sacrificed for speed. QuicKits maintain similar standards for quality that you have come to expect from the gold-standard Quantikine ELISA brand.
Linearity of dilution is a hallmark of the R&D Systems® Quantikine® ELISAs and QuicKits are no exception. Linearity is important for assessing matrix effects, confirming that the QuicKitTM ELISA accurately measures sample values. Poor linearity of dilution or extreme deviation from 100% indicates that matrix effects can artifactually enhance or interfere with analyte detection.
Figure 1. The IL-18 Quantikine® QuicKitTM ELISA has superior linearity to Brand A. At dilute concentrations, Brand A is susceptible to matrix effects.
Figure 2. The R&D Systems® IP-10 Quantikine® QuicKitTM has superior linearity to the Brand A counterpart.
Figure 3. The R&D Systems® G-CSF Quantikine® QuicKitTM has superior linearity to the Brand A counterpart.
In spike and recovery experiments, natural samples are spiked with the recombinant target analyte of interest to identify interference caused by sample matrices. Matrix effects occur when a substance in the sample interferes with the accurate detection of a target analyte. Matrix effects are caused by biological phenomena such as non-specific antibody binding, antibody cross reactivity or interference. Spike and recovery experiments are an important tool for assessing the accuracy of your measurement.
Figure 4. R&D Systems IL-18 Quantikine® QuicKitTM ELISAs have superior recovery to Brand A in serum and plasma.
Figure 5. R&D Systems TNF-alpha Quantikine® QuicKitTM ELISAs have superior recovery to Brand A in serum and plasma.
R&D Systems has been developing ELISA kits for over 30 years. We have a wide range of assays available, from our Quantikine colorimetric ready-to-use kits to our DuoSet Development ELISAs, spanning 12 different species and over 700 different targets. Still can’t find what you are looking for? We also use our development expertise to develop custom ELISA kits.
Not sure which ELISA kit you need for your research? Use our handy immunoassay selection tool to help you narrow down the ELISA kit that is right for you.