Recombinant Porcine IL-10 Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
693-PI-010/CF
Product Details
Citations (7)
FAQs
Reviews

Recombinant Porcine IL-10 Protein, CF Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 1.2‑6 ng/mL.
Source
E. coli-derived porcine IL-10 protein
Ser19-Asn175
Accession #
N-terminal Sequence
Analysis
Ser19
Structure / Form
Noncovalently-linked homodimer
Predicted Molecular Mass
18 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

693-PI/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

693-PI

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-10

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones which are dependent upon stimulation with antigen in the presence of antigen presenting cells (APC). The human homologue of murine IL-10 was subsequently cloned by cross-hybridization. Human and murine IL‑10 are 81% and 73% homologous at the nucleotide and amino acid level, respectively. Both murine and human IL-10 are also highly homologous with a previously uncharacterized open reading frame in the Epstein-Bar virus (EBV) genome, BCRF1. The BCRF1 gene product is now designated viral IL-10. Murine IL-10 is produced by Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, and LY-1+ (CD5+) and normal B cells. Human IL-10 has cross-species actvities and is active on mouse cells. Murine IL-10 is species-specific and does not act on human cells. Porcine IL-10 is 71% and 78% homologous with mouse and human IL-10, respectively.

IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, downregulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.

References
  1. Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.
Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

Citations for Recombinant Porcine IL-10 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Mutations in osteoprotegerin account for the CCAL1 locus in calcium pyrophosphate deposition disease
    Authors: CJ Williams, U Qazi, M Bernstein, A Charniak, C Gohr, E Mitton-Fit, A Ortiz, L Cardinal, AT Kaell, AK Rosenthal
    Osteoarthr. Cartil., 2018;0(0):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Infection of monocytes with European porcine reproductive and respiratory syndrome virus (PRRSV-1) strain Lena is significantly enhanced by dexamethasone and IL-10
    Authors: H Singleton, SP Graham, JP Frossard, KB Bodman-Smi, F Steinbach
    Virology, 2018;0(0):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Differential Effects of Escherichia coli Nissle and Lactobacillus rhamnosus Strain GG on Human Rotavirus Binding, Infection, and B Cell Immunity.
    Authors: Kandasamy S, Vlasova A, Fischer D, Kumar A, Chattha K, Rauf A, Shao L, Langel S, Rajashekara G, Saif L
    J Immunol, 2016;196(4):1780-9.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Inhibitors of Arg-Gly-Asp-Binding Integrins Reduce Development of Pancreatic Fibrosis in Mice
    Authors: B Ulmasov, BA Neuschwand, J Lai, V Monastyrsk, T Bhat, MP Yates, J Oliva, MJ Prinsen, PG Ruminski, DW Griggs
    Cell Mol Gastroenterol Hepatol, 2016;2(4):499-518.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Genome-wide analysis of antiviral signature genes in porcine macrophages at different activation statuses.
    Authors: Sang Y, Brichalli W, Rowland R, Blecha F
    PLoS ONE, 2014;9(2):e87613.
  6. Regulation of porcine plasmacytoid dendritic cells by cytokines.
    Authors: Lannes N, Summerfield A
    PLoS ONE, 2013;8(4):e60893.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  7. IFN-alpha treatment enhances porcine Arterivirus infection of monocytes via upregulation of the porcine Arterivirus receptor sialoadhesin.
    Authors: Delputte PL, Van Breedam W, Barbe F, Van Reeth K, Nauwynck HJ
    J. Interferon Cytokine Res., 2007;27(9):757-66.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay

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