Recombinant Viral CCI Fc Chimera (CHO-expressed) Protein, CF

Formulations:
Catalog # Availability Size / Price Qty
7055-CC-025/CF
Recombinant Viral CCI Fc Chimera (CHO-expressed) Protein, CF Data
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Recombinant Viral CCI Fc Chimera (CHO-expressed) Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit JE-induced chemotaxis of BaF3 mouse pro‑B cells transfected with human CCR2A. The ED50 for this effect is 0.06-0.3 μg/mL in the presence of 20 ng/mL of Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE).
Source
Chinese Hamster Ovary cell line, CHO-derived viral CCI protein
Viral CCI
(Met1-Val258)
Accession # P19063
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met17
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
52.6 kDa (monomer)
SDS-PAGE
60-66 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7055-CC/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7055-CC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

Measured by its ability to inhibit JE-induced chemotaxis of BaF3 mouse proB cells transfected with human CCR2A. The ED50for this effect is 0.06 -0.3 µg/mL in the presence of 20 ng/mL of Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCI

The family of T1/35 kDa proteins are encoded by the '35K' virulence gene of many poxviruses and are secreted from virus-infected cells. These proteins bind
CC-chemokines with high affinity and are termed viral chemokine inhibitor (vCCI). Viral CCI from various poxviruses share multiple stretches of sequence identity and eight conserved cysteine residues (1). The vaccinia virus (strain Lister) vCCI cDNA encodes a 258 amino acid (aa) protein with a putative 17 aa signal peptide (2). Vaccinia virus (strain Lister) vCCI shows greater than 90% aa sequence identity with vCCI from other orthopoxviruses and approximately 40% aa sequence identity with the leporipoxvirus T-1 proteins. vCCI binds with high affinity to many human, mouse, and rat CC-chemokines (1, 3-5). It binds to the same surfaces of these chemokines that are involved in chemokine receptor interactions (4-7). Some CC-chemokines utilize common binding surfaces on vCCI, while others interact with distinct sites on vCCI (8). vCCI inhibits CC-chemokine induced monocyte chemotaxis in vitro but does not block cellular effects induced by CXCL5/ENA-78, CXCL8/IL-8, or CXCL10/IP-10 (3-5). In vivo, vCCI restricts the infiltration of  immune cells into sites of inflammation (3, 9, 10) and also the CC-chemokine induced migration of arterial vascular smooth muscle cells (11).

References
  1. Graham, K.A. et al. (1997) Virology 229:12.
  2. Patel, A.H. et al. (1990) J. Gen. Virol. 71:2013.
  3. Buatois, V. et al. (2010) J. Immunol. 185:2544.
  4. Smith, C.A. et al. (1997) Virology 236:316.
  5. Lalani, A.S. et al. (1998) Virology 250:173.
  6. Beck, C.G. et al. (2001) J. Biol. Chem. 276:43270.
  7. Seet, B.T. et al. (2001) Proc. Natl. Acad. Sci. 98:9008.
  8. Burns, J.M. et al. (2002) J. Biol. Chem. 277:2785.
  9. Dabbagh, K. et al. (2000) J. Immunol. 165:3418.
  10. Reading, P.C. et al. (2003) J. Immunol. 170:1435.
  11. Spinetti, G. et al. (2004) Arterioscler. Throm. Vasc. Biol. 24:1397.
Long Name
CC Chemokine Inhibitor
Alternate Names
CCI

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