Luminescent immunoassays combine great sensitivity with broad dynamic range. One of the more effective luminescent indicators is Luminol, which, with proper enhancers, gives a bright and stable light emission.
Figure 1. Enhanced Luminol Reaction
Luminol (3-aminophthal hydrazide) was first synthesized as a chemiluminescent substrate in 1928. Unfortunately, the... More Info
DuoSet® ELISA Development Systems - ELISA Kits
Watch DuoSet® ELISA Protocol
DuoSet ELISA Development Systems contain the basic components required to develop a sandwich immunoassay for accurately measuring analytes in biological fluids. When complete kits are not an option, DuoSet ELISA Development Kits are an economical alternative to buying separate antibodies and protein standards.
View Full Assay Principle
View Complete Listing of... More Info
Quantikine® QuicKit™ ELISAs - Quantikine ELISA Kits
Get Quantikine quality in a fraction of the time!
Accomplish more in your day without compromising quality. The Quantikine QuicKit ELISAs provide quick, accurate quantitation of proteins in serum, plasma, and cell culture supernates. Unlike traditional ELISAs, QuicKit ELISAs have a fast, simplified protocol that only takes 90 minutes to results with just one wash step.
Higher... More Info
Custom ELISA Assay Services
Harness the unique experience and expertise of the R&D Systems Quantikine® ELISA team
Mellitus was looking for a development partner to assist the Company in converting its research assay to a commercially viable in vitro diagnostic. We had firsthand knowledge of the exquisite technical expertise residing within R&D Systems. With its... More Info
DuoSet ELISA Development Systems
In this brochure you will find advantages of DuoSet ELISAs, description of assay quality, range of assays and supplementary products. Some of the topics and tools that you can expect to find inside are:
Contain essential assay components
Economical and flexible
Large analyte selection
Careful antibody pair selection
Extensive quality control
DuoSet, DuoSet IC,... More Info
HT 8-oxo-dG ELISA Kits
The HT 8-oxo-dG ELISAs are plate-based immunoassays designed for detecting and quantifying 8-hydroxy-2’-deoxyguanosine (8-oxo-dG). 8-oxo-dG is a frequently used biomarker of oxidative DNA damage and oxidative stress. Reactive oxygen and nitrogen species generated by normal metabolic processes and a variety of environmental factors cause the hydroxylation of guanosine at the N7-C8 bond. 8-oxo-dG... More Info
Parameter Colorimetric Competitive ELISAs
Complete Listing of Parameter Assay Kits
The same quality and performance researchers have come to expect using our Quantikine® line of ELISA Kits has now been applied to R&D Systems Parameter Kits. These kits are complete microplate-based assays designed to accurately measure the levels of small molecules including cAMP, cGMP, NO, eicosanoids, creatinine, and more.
Fully... More Info
Supplemental ELISA Development Products
Complete Listing of Supplemental ELISA Development Products
Clear Microplates (Catalog # DY990)
A 25 pack of clear high-binding, flat-bottom polystyrene microplates (360 µL/well). Each 96-well microplate consists of 12 removable strips of 8 wells and a frame.
Black Microplates (Catalog # DY991)
A 25 pack of black high-binding, flat-bottom polystyrene microplates (360 µL/well) for use in... More Info
Troubleshooting Guide: ELISA Development
For further assistance, please contact our technical service department.
Too much signal - whole plate turned uniformly blue
Standard curve achieved but poor discrimination between points
Poor assay to assay reproducibility
No signal when a signal is expected, but standard curve looks fine
Samples are reading too high, but standard... More Info
Quantikine® HS Colorimetric Sandwich ELISA Kits - Quantikine ELISA Kits
Complete Listing of Quantikine HS ELISA Kits
Quantikine High Sensitivity ELISA Kits are complete assays generally used when very low levels of the target protein are expected. These kits utilize distinct protocols to achieve their impressive levels of analyte detection.
Detect femtogram levels of protein
Complete ready-to-use kits
Exhaustively tested for superior quality... More Info