Chemiluminescent ELISAs
Article
Luminescent immunoassays combine great sensitivity with broad dynamic range. One of the more effective luminescent indicators is Luminol, which, with proper enhancers, gives a bright and stable light emission.
Figure 1. Enhanced Luminol Reaction
Luminol (3-aminophthal hydrazide) was first synthesized as a chemiluminescent substrate in 1928. Unfortunately, the...
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DuoSet® ELISA Development Systems - ELISA Kits
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Watch DuoSet® ELISA Protocol
DuoSet ELISA Development Systems contain the basic components required to develop a sandwich immunoassay for accurately measuring analytes in biological fluids. View Full Assay Principle. When complete kits are not an option, DuoSet ELISA Development Kits are an economical alternative to buying separate antibodies and protein standards.View Complete Listing of...
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Quantikine® QuicKit™ ELISAs - Quantikine ELISA Kits
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Get Quantikine quality in a fraction of the time!
Accomplish more in your day without compromising quality. The Quantikine QuicKit ELISAs provide quick, accurate quantitation of proteins in serum, plasma, and cell culture supernates. Unlike traditional ELISAs, QuicKit ELISAs have a fast, simplified protocol that only takes 90 minutes to results with just one wash step.
Features
Higher...
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Custom ELISA Assay Services
Services
Harness the unique experience and expertise of the R&D Systems Quantikine® ELISA team
Testimonial
Mellitus was looking for a development partner to assist the Company in converting its research assay to a commercially viable in vitro diagnostic. We had firsthand knowledge of the exquisite technical expertise residing within R&D Systems. With its...
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DuoSet ELISA Development Systems
Brochures
In this brochure you will find advantages of DuoSet ELISAs, description of assay quality, range of assays and supplementary products. Some of the topics and tools that you can expect to find inside are:
Contain essential assay components
Economical and flexible
Large analyte selection
Highly referenced
Careful antibody pair selection
Extensive quality control
DuoSet, DuoSet IC,...
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HT 8-oxo-dG ELISA Kits
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The HT 8-oxo-dG ELISAs are plate-based immunoassays designed for detecting and quantifying 8-hydroxy-2’-deoxyguanosine (8-oxo-dG). 8-oxo-dG is a frequently used biomarker of oxidative DNA damage and oxidative stress. Reactive oxygen and nitrogen species generated by normal metabolic processes and a variety of environmental factors cause the hydroxylation of guanosine at the N7-C8 bond. 8-oxo-dG...
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Parameter Colorimetric Competitive ELISAs
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Complete Listing of Parameter Assay Kits
The same quality and performance researchers have come to expect using our Quantikine® line of ELISA Kits has now been applied to R&D Systems Parameter Kits. These kits are complete microplate-based assays designed to accurately measure the levels of small molecules including cAMP, cGMP, NO, eicosanoids, creatinine, and more.
Features
Fully...
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Supplemental ELISA Development Products
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Complete Listing of Supplemental ELISA Development Products
Clear Microplates (Catalog # DY990)
A 25 pack of clear high-binding, flat-bottom polystyrene microplates (360 µL/well). Each 96-well microplate consists of 12 removable strips of 8 wells and a frame.
Black Microplates (Catalog # DY991)
A 25 pack of black high-binding, flat-bottom polystyrene microplates (360 µL/well) for...
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Troubleshooting Guide: ELISA Development
Protocols
For further assistance, please contact our technical service department.
High background
No signal
Too much signal - whole plate turned uniformly blue
Standard curve achieved but poor discrimination between points
Poor duplicates
Poor assay to assay reproducibility
No signal when a signal is expected, but standard curve looks fine
Samples are reading too high, but standard...
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Quantikine® HS Colorimetric Sandwich ELISA Kits - Quantikine ELISA Kits
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Complete Listing of Quantikine HS ELISA Kits
Quantikine High Sensitivity ELISA Kits are complete assays generally used when very low levels of the target protein are expected. These kits utilize distinct protocols to achieve their impressive levels of analyte detection.
Features
Detect femtogram levels of protein
Complete ready-to-use kits
Exhaustively tested for superior quality...
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