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"Isotype Controls" has 50 results in Resources.

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The Importance of IHC/ICC Controls

Protocols

Appropriate controls are critical for the accurate interpretation of IHC/ICC results. A satisfactory IHC/ICC experimental design produces results that demonstrate that the antigen is localized to the correct specialized tissues, cell types, or subcellular location. Optimization of fixation, blocking, antibody incubation, and antigen retrieval steps will generate a strong and specific signal....

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PerCP-conjugated Antibodies

Product Highlights

Peridinin Chlorophyll Protein Complex (PerCP)-conjugated antibod­ies are convenient tools for use in flow cytometry experiments. PerCP is a water soluble carotenoid pigment found in photosynthetic dino­flagellates. It is excited by a 488 nm argon laser, and with a relatively large Stokes shift, emits at a maximum wavelength of 675 nm. Be­cause of these spectral characteristics, there is...

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Technical Notes: Intracellular Cytokine Staining

Article

Figure 1. The use of the isotype control alone (yellow) may lead to an overestimation of the number of cells staining positive for intracellular IL-1 beta. The different staining patterns between permeabilized (red) and non-permeabilized (blue) cells represents intracellular cytokine staining. Method: Peripheral blood (PMN) cells were stained for IL-1 beta after culture with 0.5 µg/mL...

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Multi-color Flow Cytometry Kits

Product Categories

Complete Listing of Multi-Color Flow Cytometry Kits Flow cytometry kits designed for analysis of cell populations use up to four fluorochrome-conjugated antibodies. Product Summary Flow cytometry is a powerful tool that is capable of rapidly measuring several cellular parameters simultaneously. Because each cell is assessed individually, the technique excels in experiments designed to...

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Technical Note: IL-17 Family Antibodies for Flow Cytometry

Article

R&D Systems has added several new fluorochrome-conjugated antibodies for the Interleukin 17 (IL-17) family of ligands and receptors. IL-17 family ligands are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions, most notably allergic responses. The six members, IL-17, IL-17B, IL-17C, IL-17D, IL-17E (also known as IL-25), and...

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Flow Cytometry Supplemental Reagents

Product Categories

Antibody Isotype Controls A wide selection of species-specific IgG isotype controls conjugated to a variety of labels. Secondary Antibodies We offer a range of secondary antibodies for flow cytometry, immunohistochemistry, and Western blotting. Staining Reagents for Flow Cytometry Flow Cytometry Staining Buffer (1X) (Catalog # FC001) For immunofluorescent staining of...

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Frequently Asked Questions

Technical Resource

To provide further customer support, we have posted answers to the most frequently asked questions about our products. Product Category Antibodies Antibody Isotype Controls Apoptosis Detection Kits & Reagents Cell Culture Products ELISAs ELISpot/FluoroSpot Kits & Development Modules Enzyme Activity Assays & Reagents Flow Cytometry Kits Luminex Assays Primer...

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Flow Cytometry Protocol for Cell Surface Markers

Protocols

Flow Cytometry Staining Overview Immunophenotyping suspended cells based on antigens present on the cell surface is one of the most common uses of flow cytometry. Since cell surface proteins are readily accessible to the antibody, a permeabilization step is not required. By staining cell surface markers, researchers can identify specific cell populations and perform fluorescence-activated...

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Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells

Protocols

Intracellular Flow Cytometry Overview Flow cytometry can be used to analyze various intracellular molecules including phosphorylated signaling proteins and cytokines. To stain intracellular molecules, the cells need to be fixed in suspension and then permeabilized before the detection antibody is added. This fixation/permeabilization treatment allows the antibody to pass through the plasma...

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CryoDefend Your Stem Cells

Scientific Abstract Posters

G. Herr, Y. Tang, M. Andersen, R. Cross, W. Johnson, D.J. Owens, S. Tousey, & J. Aho ABSTRACT Efficient cryopreservation of stem cells is essential for the maintenance of consistent stem cell stocks. Many of the existing formulations of cryopreservation media rely on high percentages of poorly defined serum or albumin. Here, we demonstrate the use of CryoDefend-Stem Cells (Catalog # CCM018)...

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