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"Magpix" has 11 results in Resources.

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xMAP® Instrument Reagents

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xMAP® Instrument Reagents Instrument Reagent Pack Size Part Number MAGPIX MAGPIX Drive Fluid, 4 x 750 mL containers MAGPIX Calibration Kit MAGPIX Performance Verification Kit 4 pack 25 uses 25 uses MPXDF-4PK MPX-CAL-K25 MPX-PVER-K25 Luminex 100/200 (all versions) Sheath Fluid Sheath Concentrate Pack (20x) 20...

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Luminex Assay Principle

Technical Resource

The sample is added to a mixture of color-coded beads, pre-coated with analyte-specific capture antibodies. The antibodies bind to the analytes of interest. Biotinylated detection antibodies specific to the analytes of interest are added and form an antibody-antigen sandwich. Phycoerythrin (PE)-conjugated streptavidin is added. It binds to the biotinylated detection...

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Luminex Instrumentation: MAGPIX, Luminex 100/200 and FLEXMAP 3D

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R&D Systems Luminex Assays are compatible with all Luminex instrumentation systems. These systems can be obtained from R&D Systems. Special bundle pricing available for instruments and assays. Luminex MAGPIX Luminex 100/200 Luminex FLEXMAP 3D Learn More Learn More Learn More Affordable, simple and compact multiplexing...

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Protocol for the Characterization of Human Regulatory T Cells

Protocols

CD4+ T cell subsets exert diverse functions due to their expression of characteristic cytokines. Regulatory T (Treg) cells constitute one subset of CD4+ effector T cells that limit the development and progression of immune responses, oppose some functions of Th17 cells, and suppress the development of autoimmunity. Treg cells are characterized by the secretion of IL-9, IL-10, IL-35, and...

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Protocol for the Characterization of Human Th22 Cells

Protocols

CD4+ T cell subsets exert diverse functions due to their expression of characteristic cytokines. T helper type 22 (Th22) cells constitute one proinflammatory subset of CD4+ effector T cells that are characterized by the secretion of IL-10, IL-13, IL-22, and TNF-alpha. Th22 cells support mucosal immunity to microbial infection. Both circulating and local cell counts of Th22 cells can be...

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Protocol for the Characterization of Human Th9 Cells

Protocols

CD4+ T cell subsets exert diverse functions due to their expression of characteristic cytokines. T helper type 9 (Th9) cells constitute one subset of CD4+ effector T cells and are characterized by the secretion of IL-9, IL-10, and IL-21. Th9 cells protect against helminth infection, promote CTL and NK cell mediated anti-tumor immunity, and contribute to chronic inflammation in allergic asthma,...

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A Homogeneous Multiplex Immunoassay for RTK Phosphorylation State that is Adaptable to HTS

Article

Jane Schmidt, Ph.D., Judy Haning, Shaya Anderson, & Richard K. Fuerstenberg INTRODUCTION Receptor Tyrosine Kinases (RTKs) are widely expressed transmembrane receptors for growth factors and other extracellular signaling molecules. Binding of a specific ligand leads to phospho-rylation at specific sites in the cytoplasmic tail that triggers further signal transduction processes. RTKs play...

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Protocol for the Differentiation and Characterization of Human Th1 Cells

Protocols

CD4+ T cell subsets exert diverse functions due to their expression of characteristic cytokines. T helper type 1 (Th1) cells constitute one subset of CD4+ effector T cells that promote cell-mediated immune responses against intracellular viral and bacterial pathogens. Th1 cells are characterized by the secretion of IFN-gamma, IL-10, and TNF-alpha. Although Th1 cells are critical for the...

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Protocol for the Differentiation and Characterization of Human Th17 Cells

Protocols

T helper type 17 (Th17) cells are a subset of CD4+ effector T cells that promote cell-mediated immune responses against extracellular bacteria and fungi. Th17 cells secrete TNF-alpha, IL-6, IL-9, IL-17A, IL-17F, IL-21, IL-22, and (human) IL-26/AK155. Related InformationTh17 Cell Interactive PathwayT Cell Subsets PosterCD4+ T Cell Subsets Brochure Differentiation of Th17 Cells See...

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Protocol for the Differentiation and Characterization of Human Th2 Cells

Protocols

CD4+ T cell subsets exert diverse functions due to their expression of characteristic cytokines. T helper type 2 (Th2) cells are a lineage of CD4+ effector T cells that provide host protection against intestinal helminths and extracellular bacteria in addition to support for B cell-dependent humoral responses. Th2 cells secrete IL-4, IL-5, IL-9, IL-13, and IL17E/IL-25. Pathological Th2 cell...

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