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"Phycoerythrin" has 20 results in Resources.

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PerCP-conjugated Antibodies

Product Highlights

Peridinin Chlorophyll Protein Complex (PerCP)-conjugated antibod­ies are convenient tools for use in flow cytometry experiments. PerCP is a water soluble carotenoid pigment found in photosynthetic dino­flagellates. It is excited by a 488 nm argon laser, and with a relatively large Stokes shift, emits at a maximum wavelength of 675 nm. Be­cause of these spectral characteristics, there is...

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Technical Notes: R&D Systems Is With You Every Step of the Way - Analyzing Proteins of Interest

Article

Step 1Use one of our Proteome Profiler™ Arrays as a screening tool to identify proteins of interest View Larger Image R&D Systems Human Cytokine Array Kit, Panel A (Catalog # ARY005) utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 36 cytokines, chemokines and acute phase proteins in a single sample....

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Analysis of Cell Viability using 7-Amino Actinomycin D (7-AAD)

Protocols

Flow cytometry provides a rapid and reliable method to quantify viable cells in a cell suspension. Determination of cell viability is critical when evaluating the physiological state of cells, such as in response to cytotoxic drugs and environmental factors, or during the progression of cancer and other disease states. In addition, it is often necessary to detect dead cells in a cell suspension...

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Flow Cytometry Protocol for Analysis of Cell Viability using Propidium Iodide

Protocols

Flow cytometry provides a rapid and reliable method to quantify viable cells in a cell suspension. Determination of cell viability is critical when evaluating the response to cytotoxic drugs or other environmental factors. In addition, it is often necessary to detect dead cells in a cell suspension in order to exclude them from the analysis. Dead cells can generate artifacts as a result of...

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NorthernLights™ Fluorescent Secondary Antibodies & Streptavidin Conjugates

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NorthernLights™ fluorescent secondary antibodies and streptavidin conjugates are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy (Figure 1)....

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NorthernLights™ Fluorescent Secondary Antibodies

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With more than 6000 primary antibodies available, the addition of the NorthernLights line of fluorescent secondary antibodies makes R&D Systems your total source for immunofluorescence reagents. NorthernLights Antibody/Label Catalog # Abs/Em Maxima Laser (Ex) Comparable Fluorochromes NL-493 anti-Rabbit IgG NL-493 anti-Mouse IgG NL-493 anti-Goat IgG NL-493...

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Technical Note: Using NorthernLights Fluorescent Secondary Antibodies for Neuronal Tracing

Article

Immunofluorescence histochemistry is a powerful tool frequently used in neuroscience for qualitative and quantitative morphological and anatomical studies. Because samples are subjected to prolonged examination under conventional fluorescence microscopes and multiple laser-scanning confocal microscopes, it is critical for fluor­escent tags to have a low susceptibility to photo­bleaching....

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Improved Expansion of MSC Without Loss of Differentiation Potential

Scientific Abstract Posters

Anissa S.H. Chan, Electra Coucouvanis, Susan Tousey, Marnelle D. Andersen and Jessie H.T. Ni ABSTRACT Mesenchymal stem cells (MSC) hold significant promise for tissue engineering and regenerative medicine. Clinical and pre-clinical applications of MSC require that they be expanded in culture with maximum efficiency while maintaining the ability to differentiate into key lineages. Fetal...

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Multiplex Measurement of Biomarkers in Human Breast Cancer Cell Culture Supernates

Scientific Abstract Posters

Jane Schmidt, Ph.D., Michael Hall, & Richard Fuerstenberg ABSTRACT The mixture of biomarkers that is produced in the tumor micro-environment has an important role in cancer pathogenesis. For example, cytokines that are released in response to infection, inflammation, or immunity can function to either promote or inhibit tumor development and progression. The multiplex immunoassay has become...

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Products in Allergy & Asthma

Product Citations

Joubert, P. et al. (2008) Expression and regulation of CCR1 by airway smooth muscle cells in asthma. J. Immunol. 180:1268. Human CCL3/MIP-1 alpha Biotinylated Fluorokine® Kit (Catalog # NFLD0) Sample: Human primary airway smooth muscle cells Application: Receptor detection by flow cytometry Human CCR1 Monoclonal Antibody (Catalog # MAB145) Sample: Human lung Application:...

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